摘要
为了解不同山茶属植物花粉的生理特性,找到最佳的萌发时间和萌发条件,以便为提高杂交育种工作效率、合理搭配杂交组合提供有益借鉴。本研究以浙江红花油茶、越南油茶、山茶、小果油茶4种山茶属植物为材料,分别统计其单花雄蕊数及花粉粒数,并重点比较了3种不同植物激素6-BA、GA_(3)、ABA处理对花粉萌发及花粉管生长的影响。结果表明,不同山茶属植物花粉萌发及花粉管生长最适宜的激素及最佳浓度各不相同,其中:(1)最适宜浙江红花油茶、越南油茶、山茶、小果油茶花粉萌发的激素处理依次为10 mg/L 6-BA、0.5 mg/L 6-BA、5 mg/L GA_(3)和10 mg/L GA_(3);(2)最适宜4种山茶属植物花粉管生长的激素处理依次为75 mg/L GA_(3)、4 mg/L ABA、125 mg/L GA_(3)和10 mg/L GA_(3);(3)4种山茶花初花期、盛花期、末花期花粉萌发率逐渐上升,其中浙江红花油茶三个时期花粉的萌发率均为4种植物中最高。
To improve the efficiency of crossbreeding,Camellia pollen was studied on the physiological characteristics in 4 different Camellia plants,which included Camellia chekiangoleosa,C.vietnamensis,C.japonica and C.meiocarppa.Number of stamens and pollen grains per flower were counted,pollen germination rates and pollen tube growth under different concentrations of 6-BA,GA_(3) or ABA were compared.Results showed that optimum hormones and concentrations for germination and pollen tube elongation of the 4 Camellia plants were different.The optimum hormones and concentrations for pollen germination of 4 species were 10 mg/L 6-BA,0.5 mg/L 6-BA,5 mg/L GA_(3) and 10 mg/L GA_(3),respectively.At the same time,the most suitable hormones and concentrations for pollen tube elongation were 75 mg/L GA_(3),4 mg/L ABA,125 mg/L GA_(3) and 10 mg/L GA_(3),respectively.The pollen germination rates of the 4 Camellia species in 3 periods were gradually rising,and the pollen activity were the highest at the late bloom stage.The pollen germination rates of C.chekiangoleosa were the highest at all of the 3 stages,which reached to about 70%at late bloom stage.
作者
刘林秀
曾海涛
徐皓
姚小华
LIU Lin-xiu;ZENG Hai-tao;XU Hao;YAO Xiao-hua(Shaanxi University of Technology,Hanzhong 723001,China;Research Institute of Subtropical Forestry,Chi-nese Academy of Forestry,Hangzhou 311400,China)
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2021年第4期700-707,共8页
Chinese Journal of Oil Crop Sciences
基金
国家重点研发计划(2019YFD1001602)
陕西省科技厅农业科技创新与攻关项目(2016NY-007)
陕西省教育厅专项科研计划项目(16JK1144)。
关键词
山茶属植物
花粉萌发
植物激素
花粉粒数
花药数
Camelliaplants
pollen germination
phytohormone
pollen grain number
anther number
