摘要
本研究以丽格海棠(Begonia×hiemalis)‘Barkos’品种嫩叶为试材,进行组培快繁关键技术的探讨。结果表明,最佳的外植体消毒方法是用0.05%NaClO浸泡10 min流水冲洗30 min,重复上述步骤2次后经Hg Cl2处理8 min。消毒外植体在培养基1/2MS+1 mg/L 6-BA+0.5 mg/L NAA中,能够迅速诱导出大量优质的不定芽。使用正交实验方法统计分析不定芽增殖培养条件,得到以不定芽数量和高度为指标的最优增殖培养基为1/2MS+1 mg/L 6-BA+0.5 mg/L NAA+150 mg/L肌醇,以继代系数为指标的最优培养条件为1/2MS+0.5 mg/L 6-BA+0.5 mg/L NAA+150 mg/L肌醇。将高度达到3 cm左右的幼苗转入1/2MS+0.5 mg/L NAA培养基中生根率可达97.2%。
The key technology of tissue culture and rapid propagation of Begonia×hiemalis ’Barkos’ was studied with an explant of leaves. The results showed that the best method of explant disinfection was to soak in 0.05%NaClO for 10 min, rinse with running water for 30 min, repeat the above steps twice and then treat with Hg Cl2 for8 min. A large number of high-quality adventitious buds were rapidly induced within the medium of 1/2 MS+1 mg/L6-BA+0.5 mg/L NAA. The multiplications of adventitious buds were analyzed by orthogonal experiment under different demands. 1/2 MS+1 mg/L 6-BA+0.5 mg/L NAA+150 mg/L inositol was the optimum medium for multiplication with high number and height. 1/2 MS+0.5 mg/L 6-BA+0.5 mg/L NAA+150 mg/L inositol was the optimum medium for multiplication with large subculture coefficient. The rooting rate reached 97.2% when the buds which were over 3 cm transferred to the medium of 1/2 MS+0.5 mg/L NAA.
作者
梁峥
贾民隆
张雨
李永平
王云山
曹冬梅
Liang Zheng;Jia Minlong;Zhang Yu;Li Yongping;Wang Yunshan;Cao Dongmei(Institute of Horticulture,Shanxi Academy of Agricultural Sciences,Taiyuan,030001;College of Horticulture,Shanxi Agricultural University,Jinzhong,030800)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第24期8206-8216,共11页
Molecular Plant Breeding
基金
山西省重点研发计划项目(201803D221005-6)
山西省科技成果转化引导专项二批(201804D131058,2016-04D132045)共同资助。
