摘要
目的:研究3D打印Ti-6Al-4V多孔支架碱酸热处理后负载黄芩苷(BC)涂层的机械性能和对细胞生物学特性的影响。方法:3D打印制备Ti-6Al-4V多孔支架并进行处理。支架材料分为空白对照组、碱酸热组、空白负载组和碱酸热负载组(实验组)。分析支架表面形貌、涂层释放效率。在各组支架材料表面接种培养大鼠骨髓间充质干细胞(BMSCs),检测细胞增殖情况及成骨相关基因表达情况。结果:3D打印Ti-6Al-4V多孔支架弹性模量是(644.00±38.47)MPa,对其处理后细胞增殖良好;培养7 d时实验组碱性磷酸酶活性(ALP)水平、骨涎蛋白(BSP)和骨钙素(OCN)mRNA水平均高于对照组(P<0.05)。结论:3D打印Ti-6Al-4V多孔支架具有与人体骨匹配的弹性模量,支架经碱酸热处理并负载BC涂层后具有良好的生物相容性,对BMSCs的成骨分化有促进作用。
Objective:To investigate the mechanical properties and biocompatibility of 3D-printed Ti-6Al-4V porous scaffold treated with alkali-acid-heat and baicalin coating(BC).Methods:The Ti-6Al-4V porous scaffold was prepared by 3D printing.The modulus of elasticity was measured.Then the scaffolds were treated with alkali-acid heat treatment and loaded with BC coating.The surface morphology of the samples was observed with SEM.The emission of BC was measured.Rat bone marrow mesenchymal stem cells(BMSCs)were cultured on the surface of the samples of blank control,alkai-acid heat treated,blank coating and alkali-acid heat with BC coating(experimental)groups,the proliferation,ALP activity and the relative mRNA expression levels of bone sialoprotein(BSP)and osteocalcin(OCN)were detected.Results:The elastic modulus of Ti-6Al-4V porous scaffold was(644.00±38.47)MPa.Ti-6Al-4V porous scaffold was successfully modified by alkali-acid heat and loaded with BC.The number of BMSCs in each group gradually increased with the culture time.The ALP level of BMSCs of experimental group were higher than those of the control groups at the 7th day(P<0.05).The mRNA relative expression level of BSP and OCN in the experimental groups was higher than that of the control group at the 7th day(P<0.05).Conclusion:The 3D-printed Ti-6Al-4V porous scaffold has suitable elastic modulus similar to human bone.The composite scaffold has good biocompatibility to promote the osteogenic differentiation of BMSCs.
作者
李伶俐
王之发
李潇
LI Lingli;WANG Zhifa;LI Xiao(Jinan University School of Stomatology,510632 Guangzhou,China;Department of Stomatology,General Hospital of Southern Theatre Command of PLA)
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2020年第6期850-854,共5页
Journal of Practical Stomatology
基金
国家自然科学基金(编号:81700943)
2016年度全军后勤科研计划项目(编号:CGZ16C006)。
