摘要
目的:建立一种简单、可靠的符合肝阳上亢证的颈性眩晕大鼠模型,同时建立一种简便可行的反应动物眩晕程度的评价方法。方法:选用SPF级雄性SD大鼠24只,8周龄,体重280~320 g,随机进行分组(每组6只),使用局部注射聚桂醇注射液(硬化剂)与附子汤灌胃相结合的方法制备肝阳上亢证颈性眩晕大鼠模型(联合造模组),与单用局部注射硬化剂的大鼠(硬化剂组)、单用附子汤灌胃的大鼠(附子汤组)、空白对照组进行对照,以大鼠通过一段玻璃管的时间(跑管时间)反应大鼠的眩晕程度,分别于造模前、造模后第2周、造模后第3周进行观测。结果:附子汤组与空白对照组跑管时间比较差异无统计学意义;联合造模组与硬化剂组跑管时间比较差异无统计学意义;硬化剂组与联合造模组跑管时间延长,不仅与对照组差异有统计学意义(P<0.05),且与附子汤组的差异有统计学意义(P<0.01)。造模后的跑管时间较造模前相比,差异具有统计学意义(P<0.05);比较造模后2、3周的跑管时间,差异无统计学意义(P>0.05)。结论:该造模方法能有效制成肝阳上亢证颈性眩晕大鼠模型,且跑管时间能在一定程度上反映大鼠的眩晕程度,为今后颈性眩晕的动物研究提供了一种简便可行的动物模型与眩晕的检测方法。
Objective:To establish a simple and reliable model of cervical vertigo in rats with hyperactivity of liver-yang syndrome,and to establish a simple and feasible method for evaluating the degree of vertigo in animals.Methods:SPF male SD rats(aged 8 weeks,weighing 280 to 320 g)were randomly divided into 4 groups(6 rats in each group).The model of cervical vertigo of hyperactivity of liver-yang syndrome(joint modeling group)was established by combining local injection of lauromacrogol(hardener)and receiving fuzi decoction by gavage.The joint modeling group was compared with the hardener group,the fuzi decoction group and the blank control group.The vertigo degree of rats was measured by the time of passing through a glass tube(running time)before modeling,2 weeks and 3 weeks after the established model.Results:There was no statistical difference in the running time between control group and fuzi decoction group,between joint modeling group and hardener group.The running time in the hardener group and the joint modeling group was longer than that in the control group(P<0.05),and was even longer than that in the fuzi decoction group(P<0.01).There was significant difference in running time after modeling compared with that before modeling(P<0.05);there was no significant difference in running time between 2 and 3 weeks after modeling(P>0.05).Conclusion:This method can effectively establish a rat model of cervical vertigo with hyperactivity of liver-yang syndrome,and the running time can reflect the degree of vertigo in rats to a certain extent.This experiment provides a simple and feasible animal model and detection method for research of cervical vertigo in the future.
作者
姜炳辰
沈佳莹
莫文
JIANG Bing-chen;SHEN Jia-ying;MO Wen(Department of Orthopaedics,Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200000,China)
出处
《中国骨伤》
CAS
CSCD
2020年第2期178-180,共3页
China Journal of Orthopaedics and Traumatology
基金
国家自然科学基金(编号:81603635)
上海市申康医院发展中心(编号:16CR3074B,16CR4011A)
上海市科学技术委员会(编号:16401930600,17401934400,18401903200)
上海市卫生与计划生育委员会(编号:20164Y0081,ZYKC201701003,201840010)
上海市浦东新区卫生与计划生育委员会(编号:PW2018D-07)
国家中医临床研究基地龙医学者编号:(编号:LYTD-60)
上海市体育科技“腾飞计划”项目(编号:18T009)。
关键词
颈性眩晕
病证结合
大鼠模型
Cervical vertigo
Combination of disease and syndrome
Rat model
