摘要
目的:观察康复新液对非甾体抗炎药引起的GES-1细胞形态损伤和凋亡相关蛋白变化的影响,为其临床治疗胃溃疡提供科学依据。方法:采用非甾体抗炎药制备胃黏膜上皮细胞损伤模型,MTT法测定不同浓度康复新液对其增殖和形态变化影响;Hoechst/PI染色检测凋亡发生情况;Western blot测定细胞中凋亡相关蛋白表达水平。结果:浓度过高或过低的康复新液均会对GES-1细胞的生长有一定的抑制作用,适当浓度的康复新液能一定程度减轻非甾体抗炎药引起的细胞损伤,减少凋亡细胞和死细胞数量;与非甾体抗炎药组相比较,康复新液组和非甾体抗炎药+康复新液组Bcl-2和Bax水平比值升高,差异具有统计学意义(P<0.01)。结论:康复新液能抑制非甾体抗炎药引起的GES-1细胞损伤,其作用机制可能与减轻非甾体抗炎药引起的GES-1细胞凋亡有关。
Objective:To observe the effect of Kangfuxin solution(KFXS)on morphological damage and apoptosis-related protein changes of GES-1 cells induced by NSAIDs.Methods:Injuried GES-1 cells model was prepared with NS398.Effect of different concentration of KFXS on the proliferation of GES-1 was determined by MTT assay.To observe the effect of KFXS on the morphological changes of GES-1,Hoechst/PI staining was used to detect the occurrence of apoptosis.The levels of apoptosis related proteins were detected by Western blot.Results:The KFXS with too high or too low concentration would inhibit the growth of GES-1 cells.The appropriate concentration of KFXS could reduce the cell damage caused by NSAIDs and the number of apoptotic cells and dead cells.Western blot results showed that compared with NS398 group,the ratios of NS398+KFXS group were higher,and the difference was significant(P<0.01).Conclusions:KFXS could inhibit the damage of GES-1 cells induced by NSAIDs,and its mechanism might be related to reduce apoptosis in GES-1 cells induced by NSAIDs.
作者
胡婷婷
饶朝龙
谢晓芳
唐欣
彭成
Hu Tingting;Rao Chaolong;Xie Xiaofang;Tang Xin;Peng Cheng(Chengdu University of Traditional Chinese Medicine,Chengdu,611137,China;Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China,Chengdu,611137,China)
出处
《成都中医药大学学报》
2019年第4期29-33,共5页
Journal of Chengdu University of Traditional Chinese Medicine
基金
国家自然科学基金项目(81630101)
国家发改委标准化项目(ZYBZH-C-SC-51)
四川省科技厅项目(2018JY0255)
四川省中医药管理局项目(2016ZY008)
