摘要
【目的】研究山茶‘赤丹’及其芽变品种花瓣中花青苷成分与含量,结合花色表型分析,明确其花色形成的物质基础,揭示其花青苷成分与花色关系,为山茶花色芽变育种提供依据。【方法】按照CIE L^*a^*b^*表色系法测量山茶‘赤丹’及其芽变品种花色,利用高效液相色谱-光电二极管阵列检测(HPLC-DAD)和超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF-MS)联用技术定性定量分析其花瓣中花青苷成分与含量,运用多元线性回归方法研究花青苷成分与花色之间的关系。【结果】山茶‘赤丹’及其芽变品种花瓣中共检测到7种花青苷,分别是矢车菊素-3-O-β-半乳糖苷(Cy3Ga)、矢车菊素-3-O-β-葡萄糖苷(Cy3G)、矢车菊素-3-O-[6-O-(E)-咖啡酰]-β-半乳糖苷(Cy3Ga E Caf)、矢车菊素-3-O-[6-O-(E)-咖啡酰]-β-葡萄糖苷(Cy3G E Caf)、矢车菊素-3-O-[6-O-(Z)-p-香豆酰]-β-葡萄糖苷(Cy3G Zp C)、矢车菊素-3-O-[6-O-(E)-p-香豆酰]-β-半乳糖苷(Cy3Ga Ep C)和矢车菊素-3-O-[6-O-(E)-p-香豆酰]-β-葡萄糖苷(Cy3G Ep C)。山茶‘玉丹’花瓣中未检测到花青苷,山茶‘金碧辉煌’中未检测到Cy3G E Caf。【结论】山茶‘赤丹’及其芽变品种花瓣的花色随其总花青苷及主要花青苷成分含量增大而加深;粉红色和红色花瓣中主要花青苷成分为Cy3G和Cy3G Ep C,黑红色花瓣中主要花青苷成分为Cy3G和Cy3Ga;随着花瓣中Cy3Ga和Cy3G比例的增大花色加深。Cy3G和Cy3G Ep C是决定山茶‘赤丹’及其芽变品种花色的主要花青苷,其含量的增大显著增加花瓣的红色程度。
【Objective】The objective of this study was to determine anthocyanin components and contents in petals of Camellia japonica‘Chidan’and its bud mutation cultivars,and to clarify the material basis of their color formation by combining with phenotypic analysis of flower colors,then reveal their relationship between anthocyanin components and flower colors and provide the basis for the bud mutation breeding of flower colors in C.japonica.【Method】Flower colors were measured by CIE L^*a^*b^* scale,and anthocyanin components and contents were measured by high-performance liquid chromatography coupled with diode array detection(HPLC-DAD)and ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS).The relationship between flower colors and anthocyanin components was explored by multiple liner regression analyses.【Result】Seven anthocyanin components were detected in C.japonica‘Chidan’and its bud mutation cultivars,that were cyanidin-3-O-β-galactoside(Cy3Ga),cyanidin-3-O-β-glucoside(Cy3G),cyanidin-3-O-(6-O-(E)-caffeoyl)-β-galactoside(Cy3Ga E Caf),cyanidin-3-O-(6-O-(E)-caffeoyl)-β-glucoside(Cy3G E Caf),cyanidin-3-O-(6-O-(Z)-p-coumaroyl)-β-glucoside(Cy3G Zp C),cyanidin-3-O-(6-O-(E)-p-coumaroyl)-β-galactoside(Cy3Ga Ep C)and cyanidin-3-O-(6-O-(E)-p-coumaroyl)-β-glucoside(Cy3G Ep C).Anthocyanins were not detected in petals of C.japonica‘Yudan’,and Cy3G E Caf were not detected in C.japonica‘Jinbi huihuang’.【Conclusion】Flower color was intensified with the increase of the contents of main anthocyanin components and total anthocyanin of C.japonica‘Chidan’and its bud mutation cultivars.The main anthocyanin components were Cy3G and Cy3G Ep C in pink and red petals,and Cy3G and Cy3Ga in dark red petals.The increase of proportions of Cy3Ga and Cy3G in petals of C.japonica‘Chidan’and its bud mutation cultivars caused intensification of the flower colors.Cy3G and Cy3G Ep C were the main anthocyanins which determined the red color of petals in C.japonica‘Chidan’and its bud mutation cultivars,and the accumulation of their contents significantly intensified red color of the petals.
作者
李辛雷
王佳童
孙振元
殷恒福
范正琪
李纪元
Li Xinlei;Wang Jiatong;Sun Zhenyuan;Yin Hengfu;Fan Zhengqi;Li Jiyuan(Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou 311400;State Key Laboratory of Tree Genetics and Breeding Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091)
出处
《林业科学》
EI
CAS
CSCD
北大核心
2019年第10期19-26,共8页
Scientia Silvae Sinicae
基金
中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2017MB007)
国家自然科学基金项目(31470697)
林业公益性行业科研专项经费项目(201504707)
关键词
山茶
芽变
花青苷
花色
超高效液相色谱-四极杆-飞行时间质谱
Camellia japonica
bud mutation
anthocyanin
flower colors
ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry
