摘要
为研究山楂黄酮对小鼠脾淋巴细胞的免疫调节作用,将小鼠分为空白组、左旋咪唑组和不同浓度山楂黄酮处理组(100、200、400和800μg/mL)。利用MTT法检测山楂黄酮对淋巴细胞增殖的影响,ELISA法检测山楂黄酮对淋巴细胞白细胞介素-6(IL-6)、白细胞介素-4(IL-4)、干扰素-γ(IFN-γ)的影响,流式细胞术检测山楂黄酮对淋巴细胞膜表面标志物(CD4+和CD8+)的影响。结果表明,山楂黄酮在100~400μg/mL对淋巴细胞增殖和淋巴细胞因子的分泌具有浓度依赖性,IL-6和IFN-γ的分泌极显著高于空白组(P<0.01),IL-4的分泌极显著低于空白组(P<0.01),且400μg/mL处理组与左旋咪唑组无统计学意义(P>0.05);对淋巴细胞亚群CD4+CD8-、CD4-CD8+具有浓度依赖性的促进作用,且极显著高于空白组、左旋咪唑组(P<0.01);对淋巴细胞亚群CD4+/CD8+百分比极显著高于空白组、左旋咪唑组(P<0.01),200μg/mL浓度组CD4+/CD8+的比值极显著高于其它浓度组(P<0.01)。100~400μg/mL山楂黄酮通过诱导淋巴细胞增殖和淋巴细胞因子IL-6、IL-4和IFN-γ的分泌,提高淋巴细胞亚群CD4+/CD8+比值,从而发挥免疫调节作用。
To study the immunomodulatory effect of hawthorn flavonoids on spleen lymphocytes in mice,mice were divided into blank group,levamisole group and hawthorn flavonoids treatment group(100,200,400 and 800μg/mL).MTT method was used to detect the effect of hawthorn flavonoids on lymphocyte proliferation.ELISA method was used to detect the effect of hawthorn flavonoids on lymphocyte interleukin-6(IL-6),interleukin-4(IL-4),interferon-γ(IFN-γ).Flow cytometry was used to detect the effect of hawthorn flavonoids on lymphocyte membrane surface markers(CD4+and CD8+).Results showed that hawthorn flavonoids had concentration dependence on lymphocyte proliferation and lymphocyte factor secretion ranged from 100 to 400μg/mL.The secretion of IL-6 and IFN-γwere higher than that of the blank group extremely significant(P<0.01),and the secretion of IL-4 was lower than that of the blank group extremely significant(P<0.01),and there was no statistical significance between the 400μg/mL treatment group and levamisole group(P>0.05).The percentage of CD4+CD8-and CD4-CD8+in lymphocyte subsets had a centration-dependent promoting effect,which was higher than that in blank group and levamisole group extremely significant(P<0.01).The percentage of CD4+/CD8+in lymphocyte subsets was higher than that in blank group and levamisole group extremely significant(P<0.01),and the ratio of CD4+/CD8+in 200μg/mL treatment group was higher than that in other concentration groups(P<0.01).Hawthorn flavone could enhance the CD4+/CD8+ratio of lymphocyte subpopulation by inducing lymphocyte proliferation and secretion of lymphocyte factors IL-6,IL-4 and IFN-γranged from 100 to 400μg/mL,and it played an immunomodulatory role.
作者
王一伦
李敬双
李美莹
于洋
WANG Yi-lun;LI Jing-shuang;LI Mei-ying;YU Yang(College of Food Science and Engineering,Jinzhou Medical University,Jinzhou 121001,China)
出处
《食品工业科技》
CAS
北大核心
2019年第20期127-132,145,共7页
Science and Technology of Food Industry
基金
辽宁省自然科学基金(20170540369、20170540357)
2018年辽宁省大学生创新创业训练计划项目(201810160014)
关键词
山楂黄酮
淋巴细胞增殖
细胞因子
淋巴细胞膜表面标志
免疫调节
hawthorn flavonoids
lymphocyte proliferation
cytokines
surface marker of lymphocyte membrane
immunoregulation
