摘要
目的探讨青蒿琥酯(artesunate, ASN)对结核分枝杆菌(mycobacterium tuberculosis, Mtb)诱导THP-1细胞分泌TNF-α和IL-6的影响及机制研究。方法体外培养THP-1细胞。MTT法检测ASN对细胞的活性影响;RT-qPCR检测细胞因子TNF-α、IL-6和TLR2mRNA表达;ELISA检测TNF-α、IL-6分泌,Western blot检测TLR2受体表达。结果 MTT法检测ASN浓度在0～20μg/mL范围内培养48h均对细胞无毒性;与对照组相比,Mtb显著增加TNF-α、IL-6mRNA及蛋白表达,而20μg/mL ASN能显著抑制TNF-α、IL-6 mRNA及蛋白表达(P<0.05)。此外,20μg/mLASN能显著抑制TLR2mRNA及蛋白表达。结论 ASN可能通过抑制TLR2受体表达缓解Mtb诱导的炎症反应。
Objective To investigate the effect of artesunate(ASN)on the secretion of cytokine TNF-α and IL-6 in THP-1 cells induced by Mtb and to investigate its potential underline mechanism. Methods THP-1 cells were cultured in vitro.MTT assay was used to detect the effect of ASN on the activity of cells.RT-qPCR was used to detect the mRNA expressions of TNF-α,IL-6 and TLR2.ELISA was used to detect the secretion of TNF-α and IL-6 protein.Western blot was used to detect the protein expression of TLR2. Results There was no cytotoxicity to cell dealth with the concentrations of 0-20ug/ml of ASN during 48hculture.Compared with the control group,Mtb significantly increased mRNA and protein expressions of TNF-α and IL-6.ASN could inhibit the secretion of TNF-α and IL-6 at mRNA and protein levels( P <0.05).In addition,ASN could down-regulate the expressions of TLR2 mRNA and protein. Conclusion ASN may inhibit the inflammatory responses induced by Mtb through the down-regulation of TLR2 expression.
作者
马小华
刘爱凤
向延根
刘栋宾
潘建华
喻容
石国民
MA Xiao-hua;LIU Ai-feng;XIANG Yan-gen;LIU Dong-bin;PAN Jian-hua;YU Rong;SHI Guo-min(Department of Clinical Laboratory,Changsha Central Hospital,Hunan Province,Changsha 410004,China;Department of Pathology,Changsha Central Hospital,Hunan Province,Changsha 410004,China)
出处
《标记免疫分析与临床》
CAS
2019年第2期338-342,共5页
Labeled Immunoassays and Clinical Medicine
基金
湖南省卫生和计生委员会科研课题(编号:B2016156)
