摘要
目的 探讨子宫内膜癌和卵巢上皮性癌组织中抑癌基因PTEN的突变及其蛋白表达与肿瘤发生、发展的关系。方法 应用聚合酶链反应 单链构象多态性分析 (PCR SSCP)和DNA序列分析法 ,检测子宫内膜癌 (5 2例 )、卵巢上皮性癌 (6 0例 )中PTEN基因第 5和第 8外显子的突变 ,免疫组织化学法检测PTEN蛋白的表达。结果 子宫内膜癌组织 :(1)PTEN基因突变率为 2 5 % ,高于正常子宫内膜 (0 ,P <0 0 5 ) ;PTEN基因突变率与子宫内膜癌的病理分级、组织类型、肌层浸润深度密切相关(P <0 0 5 )。(2 )PTEN蛋白缺失率为 6 0 % ,高于正常子宫内膜组织 (0 ,P <0 0 5 ) ;子宫内膜癌组织中PTEN蛋白表达率与病理分级和组织类型密切相关 (P <0 0 5 ) ,而与手术病理分期和肌层浸润深度无关 (P >0 0 5 )。卵巢上皮性癌组织 :(1)PTEN基因的突变率为 5 % ,与正常卵巢组织 (0 )和良性卵巢肿瘤 (0 )相比 ,差异无显著性 (P >0 0 5 )。 (2 )PTEN蛋白缺失率为 2 3% ,显著高于良性卵巢肿瘤和正常卵巢组织 (均为 0 ,P <0 0 5 ) ;卵巢上皮性癌组织中的PTEN蛋白缺失率与其组织类型及患者的年龄、绝经与否无明显相关性 (P >0 0 5 ) ,与临床分期和病理分级有关 (P <0 0 5 )。
Objective To study the relationship between the mutation and protein expression of PTEN and carcinogenesis of endometrial carcinoma and epithelial ovarian cancer. Methods The mutations of Exon5,8 of PTEN in epithelial ovarian cancer tissues and endometrial carcinoma tissues were examined by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequence analysis; and immunohistochemistry was used to evaluate the expression of PTEN protein in corresponding tissues. Results The mutation rate(25%) in endometrial carcinomas was higher than that of normal tissue(0, P <0.05) and the mutation rate had significant relationship with pathological grade ,histological type and depth of myometrial invasion( P <0.05). PTEN protein expression deletion rate (60%) in endometrial carcinoma tissues was higher than that of normal tissue (0, P <0.05). The protein expression deletion was significantly associated with pathological grade and histological type( P <0. 05),but was irrelevant to stage and depth of myometrial invasion ( P >0.05). The mutation frequency in epithelial ovarian carcinomas(5%) had no significant difference compared with those of normal ovarian tissues(0) and benign epithelial ovarian tumors(0). PTEN protein expression deletion in epithelial ovarian cancers (23%) was higher than those of normal ovarian tissues (0) and benign epithelial ovarian tumors (0, P <0.05). The expression level of PTEN protein varied between different stages and differential grades. There was no difference between the expression levels of two different histological types and different ages. Conclusion The nutation and expression deletion of PTEN played a role in the carcinogenesis of endometrial cancers, however, in epithelial ovarian cancers PTEN gene played its role by expression deletion other than mutation.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2002年第10期608-611,共4页
Chinese Journal of Obstetrics and Gynecology
关键词
蛋白表达
基因突变
聚合酶链反应-单链构象多态性分析
子宫内膜肿瘤
卵巢肿瘤
Endometrial neoplasms
Ovarian neoplasms
Mutation
Genes, suppressing, tumor
Phosphoric monoseter hydrolases
Tumor suppressor proteins
