摘要
目的优化育亨宾树皮中育亨宾的提取工艺。方法以育亨宾为测定指标,建立HPLC检测方法;通过单因素考察了不同溶剂、酸水体积分数、料液比、提取时间和提取温度对育亨宾提取率的影响;在单因素实验的基础上,采用正交实验L_9(3~4)确定最佳提取工艺。结果育亨宾质量浓度在10~800μg·mL^(-1)(r=0.999 9)范围内呈良好的线性关系;最佳提取工艺为22倍质量分数0.2%的盐酸水溶液,100℃提取3次,每次1.5h。结论该提取方法简单可行,可得到质量均一稳定的育亨宾提取物。
Objective To optimize the extraction process of yohimbine from yohimbine bark yohimbine. Methods HPLC detection method was established for yohimbine simultaneous determination.The effects of different solvents,acid water volume fraction,solid-liquid ratio,extraction time and extraction temperature on the extraction rate were investigated.On the basis of single factor experiment,the optimal extraction process was determined by an orthogonal test L 9（3 4）. Results Yohimbine showed a good linerity in the range of 10-800μg·mL^-1（ r =0.999 9）;the optimal extraction conditions were concluded as follows：22 times amount of 2 mL·L^-1hydrochloric acid water as extractant,extraction 3 times （each time 1.5 h） and extraction temperature 100 ℃. Conclusion It was simple,stable and suitable for mass yohimbine production.
作者
张敏
闫高颖
张必荣
王平
任晓峰
ZHANG Min;YAN Gaoying;ZHANG Birong;WANG Ping;REN Xiaofeng(Shaanxi Tiangu Biological Technology Group Limited Company,Xi′an 710065,China;Hanzhong Tianrangu Biological Technology Group Limited Company,Hanzhong 723100,China)
出处
《西北药学杂志》
CAS
2018年第5期588-591,共4页
Northwest Pharmaceutical Journal
基金
汉中市植物提取产业研发中心天然产物研发分中心创新研发能力建设项目(汉市科创发[2016]1号)
