摘要
目的建立同时测定萝芙木中的5种吲哚类生物碱含量的方法。方法采用Diamonsil C_(18)色谱柱(250 mm×4.6 mm,5μm),柱温25℃,流动相为甲醇-水(梯度洗脱),流速1 m L·min^(-1),检测波长280 nm,进样量5μL。结果萝芙木中蛇根精、育亨宾、萝芙木碱、阿马里新、利血平5种吲哚类生物碱在1.56~200.0μg·m L^(-1)范围内线性关系良好,相关系数均大于0.999 0,5种吲哚类生物碱的平均回收率为95.0%~105.0%(n=9)。结论该方法简单,结果准确、重复性好,可用于萝芙木药材的质量控制。
Objective To establish an high perfermance liquid chromatography method for determination of five indole alkaloids in Rauvolfia. Methods The Diamonsil C18 column was used at 25 with methanol-water （gradient elution） as the mobile phase, the flow rate was 1. 0 mL＊min 1,the detective wavelength was 280 nm, and the injection volume was 5 ml·L^-1. Results In the range of 1. 56 - 200 μg·m L^-1, the correlation coefficients of regression equations of sarpagine, yohimbine, ajmaline, ajmalicine, reserpine were higher than 0. 999 0. The average recovery （ n = 9 ） of five indole alkaloids was between 95. 0% - 105. 0%. Conclusion This method is simple with good accuracy and repeatability. It can be used for quality control of Rauvolfia.
出处
《医药导报》
CAS
2017年第4期425-428,共4页
Herald of Medicine
基金
齐齐哈尔市科学技术计划项目(SFZD-2013021)
关键词
萝芙木
吲哚生物碱
含量测定
质量控制
色谱法
高效液相
Rauvolfia
Indole alkaloids
Content determination
Quality control
Chromatography, high performance liquid
