摘要
目的 探讨3种手术用冲洗液在38 ℃手术室恒温箱内的安全存放时间。方法 2016年3— 12 月选择3 种手术用冲洗液:500 ml 瓶装0.9% 氯化钠溶液(A 类)、袋装3 000 ml 0.9% 氯化钠溶液(B 类)、袋装3 000 ml 甘露醇(C 类),每种冲洗液随机各抽取出240 袋,将每种240 袋手术用冲洗液按随机数字法分为4 组(各60 袋),其中3 组放置于38 ℃的手术室恒温箱内,存放时间分别为6 h、7 d、30 d,另一组为空白对照组(置于室温)。分别对加温存放6 h、7 d、30 d后的3 种冲洗液进行细菌学、液体的渗透压摩尔浓度、pH值检测,并观察液体外观、有无异味。数据比较采用单因素方差分析及LSD-t 检验。结果 3 种液体在加温存放6 h、7 d、30 d 后细菌培养结果均为阴性,外观无明显变化,均无异味。加热后3 种冲洗液(6 h 组、7 d 组、30 d 组)的pH值、渗透压摩尔浓度分别高于同类液体的空白对照组,差异有统计学意义(P< 0.05);但6 h组、7 d组、30 d组冲洗液的pH值及渗透压摩尔浓度差异无统计学意义(P> 0.05)。结论 在38 ℃手术室恒温箱内放置30 d 内的手术用冲洗液的细菌培养结果均为阴性,外观无明显变化亦无异味,加热后液体的渗透压摩尔浓度和pH 值稍有变化,但都在正常范围之内。
Objective To study the safe storage time of 3 kinds of irrigating fluid in incubators at 38 ℃ in operating room. Methods From March to December 2016, 3 kinds of irrigating liquid, including 500 ml bottled 0.9% Sodium Chloride Solution (A), 3 000 ml bagged 0.9% Sodium Chloride Solution (B), and 3 000 ml bagged mannitol (C) were selected. Two hundred and forty bottles (bags) were randomly taken out from each kind of liquid, and divided into 4 groups by random number table method with 60 bottles (bags) in each group. Three of the groups were heated in 38 ℃ incubators for 6 h, 7 d and 30 d, and the last group was set up as the blank control group. According to aseptic procedures, the bacteriological examination, the osmolality, the pH value, the appearance and the peculiar smell of the washing fluid at 6 h, 7 d and 30 d were investigated respectively. Single factor analysis of variance and Least Significant Difference (LSD) t-test were applied in data comparison. Results The results of bacterial cultures of the 3 kinds of liquid were negative, with no appearance change and no odor at 6 h, 7 d and 30 d. The pH and osmolality of all heated groups were all higher than the control group of the same kinds of liquid, and the differences were statistically significant (P 〈 0.05), however, there was no statistical difference in pH value and osmolality among the 6 h group, 7 d group and 30 d group (P ~ 0.05). Conclusions The results of bacterial culture of all the irrigating fluid heated in 38 incubators were negative in 30 days, with no apparent change and no peculiar smell. The osmolality and pH changed slightly, but the results were within the normal range.
作者
李莉
刘静云
Li Li;Liu Jingyun(Operating Room, the First Hospital of China Medical University, Shenyang 110001, China)
出处
《中华现代护理杂志》
2018年第4期414-417,共4页
Chinese Journal of Modern Nursing
基金
2015年中国医科大学护理学院科研项目(2015HL-04)
关键词
手术室
氯化钠
甘露醇
冲洗液
加热
PH
值
渗透压
Operating rooms
Sodium chloride
Mannitol
Irrigating fluid
Heating
pH
Osmolality pressure