摘要
目的比较等量附子不同组方的4种中药制剂（单一附子汤、姜附汤、四逆汤和参附注射液）中碱性物质的质量浓度。方法以乌头碱为对照品,通过紫外可见光谱扫描或检测,分别获得单一附子汤、姜附汤、四逆汤和参附注射液（质量浓度均为附子生药0.2 g/m L）中的碱性物质在200~600 nm波长下的吸光度（A）-波长曲线下面积（吸波面积）和415 nm下溴甲酚绿比色法的A值,计算碱性物质量,并对2种方法得到的结果进行比较。结果吸波面积法和溴甲酚绿比色法获得的结果均准确可信、方法可行,线性方程分别为吸波面积法Y=0.145 42 X＋3.240 4,r=0.999 3;溴甲酚绿比色法Y=0.008 63 X＋0.006 57,r=0.999 5;吸波面积法获得的单一附子汤、姜附汤、四逆汤和参附注射液中的整体碱性物质质量浓度分别为1 137.992、2 907.120、2 455.611、298.208μg/m L,而溴甲酚绿比色法测得的只是乌头碱类的碱性物质,质量浓度分别为293.824、447.767、343.804、38.382μg/m L;前者的质量浓度比后者的大得多,但均符合姜附汤〉四逆汤〉单一附子汤〉参附注射液的质量浓度趋势。结论等量附子不同组方的中药制剂中碱性物质的质量浓度不同,吸波面积法测得的整体碱性物质质量浓度比溴甲酚绿比色法测得的乌头碱类碱性物质质量浓度更全面,对附子中药制剂的配伍机制、量效关系和安全性研究将提供更科学的理论基础。
Objective To compare with the contents of alkaline substances from four different prescription of formulations of traditional Chinese medicine（single aconite decoction, Jiangfu Decoction, Sini Decoction, and Shenfu Injection） with the same dose of aconite. Methods Taken aconitine as the standard substance, the area under absorbance-wavelength curve（AUAWC） and absorption values of alkaline substances from four formulations, namely single aconite decoction, Jiangfu Decoction, Sini Decoction and Shenfu Injection（every concentration of aconite crude herb for 0.2 g/m L as the same dose）, were obtained, respectively, which were determined by the method of AUAWC after the scanning for the wavelength from 200~600 nm and the method of bromocresol green colorimetric at 415 nm, and the data calculated were compared. Results The results are accurate and reliable, and the methods of both AUAWC and bromocresol green colorimetric are feasible. The linear equations were Y = 0.145 42 X ＋ 3.240 4, r = 0.999 3 for the AUAWC and Y = 0.008 63 X ＋ 0.006 57, r = 0.999 5 for the bromocresol green colorimetric, respectively. The contents of alkaline substances from single aconite decoction, Jiangfu Decoction, Sini Decoction and Shenfu Injection were acquired through the method of AUAWC, which were 1 137.992, 2 907.120, 2 455.611, and 298.208 μg/m L, respectively, and the contents of aconitum alkaline substances were obtained by bromocresol green colorimetric with the concentration of 293.824, 447.767, 343.804 and 38.382 μg/m L, respectively. The content determined by the former method was much larger than that of the latter method, but the trend of the contents from two methods was the same, which was Jiangfu Decoction Sini Decoction single aconite decoction Shenfu Injection. Conclusion The contents of alkaline substances from different prescription of formulations of traditional Chinese medicine with the same dose of aconite are different, and the concentration of overall alkaline substances from AUAWC is more comprehensive than that of aconitum alkaline substances determined by bromocresol green colorimetric, which will provide more scientific theory interpretation for compatibility mechanism and dose-effect relationship as well as security research of Chinese medicine preparation of aconite.
出处
《中草药》
CAS
CSCD
北大核心
2016年第24期4364-4369,共6页
Chinese Traditional and Herbal Drugs
基金
福建省科技厅引导性项目(2017Y01010229)
关键词
附子
单一附子汤
姜附汤
四逆汤
参附注射液
吸波面积
溴甲酚绿比色法
中药制剂
碱性物质
配伍机制
量效关系
安全性
aconite
single aconite decoction
Jiangfu Decoction
Sini Decoction
Shenfu Injection
AUAWC
bromocresol green colorimetry
Chinese material medica preparation
alkaline substances
compatibility mechanism
dose-effect relationship
safety
