摘要
目的:考察全自动微粒子化学发光免疫实验(CMIA)与TP-Ig M抗体检测(TP-ELISA)在梅毒检测中的应用效果差异。方法:采用TPPA法作为金标准检测1000例住院患者血清标准,评价CMIA与TP-ELISA在梅毒检测中敏感性和特异性。结果:金标准中1000例血清标本梅毒检出率为12.3%,CMIA阳性检出率为12.9%,TP-Ig M为11.7%。CMIA法灵敏度为98.4%(121/123),特异性为99.1%(869/877),阳性预测值为93.8%(121/129),阴性预测值为99.8%(869/871)。TP-ELISA法灵敏度为89.4%(110/123),特异性为99.2%(870/877),阳性预测值为94.0%(110/117),阴性预测值为98.5%(870/883)。两组间灵敏度和阴性预测值存在显著差异(P<0.05)。结论:CMIA的灵敏性和阴性预测值优于TP-ELISA法,可以替代TP-PA成为梅毒检测的血清学检测首选方法。
Objectives： To investigate the effect of automatic particle chemiluminescence immunoassay（ CMIA） and TP- Ig M antibody（ TP- ELISA） in the detection of syphilis. Methods： Serum sample of 1000 hospitalized patients were detected by TPPA as gold standard,and the sensitivity and specificity of CMIA and TP-ELISA in the detection of syphilis were evaluated. Results： The positive rate of syphilis was 12. 3% in 1000 patients. The positive rates of CMIA and TP- Ig M were 12. 9% and11. 7% respectively. The sensitivity and specificity of CMIA was 98. 4%（ 121 /123） and 99. 1%（ 869 /877）,the positive predictive value was 93. 8%（ 121 /129）,and the negative predictive value was 99. 8%（ 869 /871）. The sensitivity of TP- ELISA was 89. 4%（ 110 /123）,specificity was 99. 2%（ 870 /877）,the positive predictive value was 94%（ 110 /117）,and the negative predictive value was 98. 5%（ 870 /883）. There were significant differences between the two groups in the sensitivity and the negative predictive value（ P〈0. 05）. Conclusion： The sensitivity and negative predictive value of CMIA is better than these of TP- ELISA,and it can replace TP- PA as the first choice for the detection of syphilis.
作者
屈晓威
史龙泉
冯莉莉
马利军
屈义芳
QU Xiaowei SHI Longquan FENG Lili MA Lijun QU Yifang(Clinical Laboratory, The Hospital Affiliated to Yan＇ an University, Yan＇ an 716000, Shanxi, China Dermatology Department, The Hospital Affiliated to Yan ＇ an University, Yan ＇ an 716000, Shanxi , China Yan＇ an City People＇ s Hospital, Yan＇ an 716000, Shanxi, China)
出处
《中国性科学》
2016年第12期70-72,共3页
Chinese Journal of Human Sexuality
基金
陕西省延安市科学技术研究发展计划项目(2014HM-07)
关键词
梅毒
血清学检验
CMIA
TP-ELISA
Syphilis
Serological test
Chemiluminescence immunoassay（CMIA）
TP-IgM antibody（TP-ELISA）
