摘要
目的:考察全自动微粒子化学发光免疫实验(CMIA)与免疫层析技术(GICA)在梅毒检测中的应用效果差异。方法:采用TPPA法作为金标准检测1500例住院患者血清标准,评价CMIA与GICA在梅毒检测中敏感性和特异性。结果:金标准中1500例血清标本梅毒检出率为12.1%,CMIA阳性检出率为12.5%,GICA为12.7%。CMIA法灵敏度为92.86%,特异性为98.,6%,阳性预测值为89.89%,阴性预测值为99.01%。GICA法灵敏度为98.35%,特异性为99.77%,阳性预测值为98.35%,阴性预测值为99.77%。两组间灵敏度和阴性预测值存在显著差异(P<0.05)。结论:GICA的灵敏性和阴性预测值优于GICA法,可以提到TPPA成为梅毒检测的血清学检测首选方法。
Objectives： To investigate the effect of automatic particle chemiluminescence immunoassay（ CMIA） and colloidal gold immunochromatography assay（ GICA） in the detection of syphilis. Methods： Serum sample of 1000 hospitalized patients were detected by TPPA as gold standard,and the sensitivity and specificity of CMIA and GICA in the detection of syphilis were evaluated. Results： The positive rate of syphilis was 12. 1% in1500 patients; the positive rate of CMIA was 12. 5%; GICA was 12. 7%. The sensitivity of CMIA was 92. 8%;specificity was 98. 5%; the positive predictive value was 93. 8% and the negative predictive value was 99. 0%. The sensitivity of GICA was 98. 4%; specificity was 99. 7%; the positive predictive value was 94. 0%,and the negative predictive value was 99. 7%. There was significant difference between the two groups in the sensitivity and the negative predictive value（ P〈 0. 05）. Conclusion： The sensitivity and negative predictive value of CMIA is better than that of GICA,and it can replace TP- PA as the first choice for the detection of syphilis.
出处
《中国性科学》
2016年第10期69-71,共3页
Chinese Journal of Human Sexuality
基金
湖北省自然科学基金项目(2012FFA071)
关键词
梅毒
血清学检验
CMIA
GICA
Syphilis
Serological test
Chemiluminescence immunoassay（CMIA）
Immunochromatography（GICA）
