摘要
采用HPLC法,以表没食子基儿茶素没食子酸酯(EGCG)为对照品,外标法测定其在茶多酚提取物中的量,并分别测定EGCG与表没食子儿茶素(EGC)、儿茶素(C)、表儿茶素(EC)、没食子基儿茶素没食子酸酯(GCG)和没食子酰表儿茶素(ECG)的相对校正因子,用获得的相对校正因子计算后5种成分的量,实现一测多评;同时,用外标法测茶多酚提取物中EGC、C、EC、GCG和ECG的量。向量夹角余弦法分析,表明外标法的测定值与采用相对校正因子的计算值之间无显著性差异,说明一测多评法可以应用于茶多酚提取物及其制剂的多指标质量评价。
In this study, a new strategy for quantitative analysis of multi-components using a single marker （QAMS） was developed and validated to analyze six catechins （epigallocatechin, catechin, epicatechin, epigallocatechin gallate, gallocatechin gallate,and epicatechin gallate） in tea polyphenols extract by HPLC. The QAMS method is based on the relative correction factor （RCF） between each analyte and single standard within certain concentration ranges. In the present study,epigallocatechin gallate was selected as the internal reference substance. The RCFs values between epigallocatechin gallate and the other five catechins were investigated using five different types of chromatographic columns. The content of other five components were calculated by RCFs, respectively. Meanwhile,the content of the six analytes was also determined by a conventional external standard method to compare with QAMS method. Finally, the angle cosine value which were all above 0. 999542 revealed that there was no significant difference between the QAMS method and the ex- ternal standard method. The QAMS method established in this study solved the problem of the availability and high cost of some standard substances, and would be useful for providing an efficient and feasible quality assessment method for tea polyphenols extract and its preparations.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2016年第10期1568-1571,1638,共5页
Natural Product Research and Development
基金
四川省2015大学生创新训练项目(201513705078)
四川省教育厅科研计划(16ZA0299)
电子科技大学新进教师科研启动基金(ZYGX2015KYQD077)
关键词
茶多酚
儿茶素
一测多评法
高效液相色谱法
tea polyphenols extract
catechins
quantitative analysis of multi-components via single marker method
high performance liquid chromatography
