摘要
目的:用HPLC-UV法建立蒙成药三子散中川楝素含量测定方法。方法:参照《中国药典》2015年版川楝子中川楝素含量测定方法,采用HPLC-UV法建立三子散中川楝素含量测定方法,测定三子散中川楝素的含量。摸索色谱条件,并开展方法学考察。结果:色谱条件确定为:采用辛烷基硅烷键合硅胶为填充剂(C8柱);以乙腈-水(40∶60)为流动相;柱温为40℃;检测波长为210 nm。方法学考察表明该方法有较好的精密度、准确度、重复性,在本研究条件下,川楝素在0.2~4μg内线性关系良好(r=0.9999),平均回收率为98.3%,RSD=0.4%(n=6)。结论:本研究建立了HPLC-UV测定蒙成药三子散中川楝素含量的方法,在该色谱条件下,色谱峰分离与色谱表现呈现对温度敏感的结果。建立的方法灵敏、快速、准确,适用于三子散中川楝素的含量测定。
Objective:To establish a method for the determination of toosendanin in Mongolian patent medicine Sanzisan by HPLC-UV.Methods:According to the extraction method of toosendanin content determination in 2015 edition of Chinese Pharmacopoeia,HPLC-UV method was used to establish the content determination method of toosendanin in Sanzisan and determine the content of toosendanin in Sanzisan.The related chromatographic conditions were explored and the methodological investigation was carried out.Results:The chromatographic conditions were as follows:octane silane bonded silica gel was used as filler(C8 column);acetonitrile water(40∶60)was used as mobile phase;column temperature was 40℃;detection wavelength was 210 nm.The results showed that the method had good precision,accuracy and repeatability.Under the condition of this study,the linear relationship of toosendanin was good(r=0.9999),the average recovery was 98.3%,RSD=0.4%(n=6).Conclusion:A HPLC-UV method was established for the determination of toosendanin in"sanzisan",a Mongolian patent medicine.Under this condition,the chromatographic peak separation and chromatographic performance showed temperature sensitive results.The method is sensitive,rapid and accurate,and is suitable for the determination of toosendanin in Sanzi powder.
作者
张建平
王栋
周雪梅
孙倩男
杜文亚
ZHANG Jian-ping;WANG Dong;ZHOU Xue-mei;SUN Qian-nan;DU Wen-ya(The Inner Mongolia Autonomous Region Institute for drug control,The Inner Mongolia Autonomous Region Key Laboratory of Standard Research of Chinese and Mongolian Medicine,Hohhot,010020)
出处
《中国民族医药杂志》
2021年第2期38-42,共5页
Journal of Medicine and Pharmacy of Chinese Minorities
