摘要
采用水蒸汽蒸馏法提取疏毛罗勒(Ocimum basilicum L)挥发油,并采用气-质联用方法分析其化学成分,再采用DPPH·清除能力测定法和O^-_2·清除能力测定法测定其抗氧化活性,杯碟法测定其抑菌活性。结果表明,疏毛罗勒挥发油组分共检出27种成分,所鉴定出化学成分的质量占总量的90.10%,其主要化学成分是十六酸乙酸乙酯(44.16%)、乙酸松油酯(17.1%)、(s)-2,5,5,8-四甲基-1,2,3,6-四氢化薁-4-酮(9.92%)、香叶醇(3.05%)等;挥发油成分对DPPH·和O^-_2·有中等程度的清除能力;其对大肠埃希菌、金黄色葡萄球菌、白色念珠菌3株菌种有一定的抑菌作用,并且浓度越高,抑菌效果越明显。
The constituents of volatile oil in the Ocimum basilicum L were analyzed by GC-MS method after it was extracted via steam distillation method.The antioxidant activity was assayed via scavenging properties of DPPH· and O^-_2·,the antibacterial activity was studied by using the cup-plate method.The results showed that 27 kinds of components were indeed recognized in volatile oils constituent.The identified ingredients accounted for nearly 90.10% of weak polarity constituents,its main chemical composition was hexadeanoic acid ethyl easter(44.16%),fenchyl acetate(17.1%),geraniol(3.05%),(s)-2,5,5,8-tetramethyl-1,2,3,6-tetrahydro-azulen-4(5H)-one(9.92%),etc.The volatile oil constituents had moderate activity for scavenging properties of DPPH·and O^-_2·.The constituents also had certain antibacterial activity against Escherichia coli,Staphylococcus aureus,Bacillus subtilis and Candida albicans,and the higher the concentration was,the more obvious the antibacterial activity was.
基金
安徽省质量工程-药学专业省级教学团队项目(2013jxtd067)
安徽省质量工程-药品质量检测技术特色专业项目(2014tszy055)
安徽省高等学校自然科学研究项目重点课题(KJ2015A358)
亳州市中药科技创新团队项目(BZ2014zzb21)
