摘要
目的探讨β绒毛膜促性腺激素的第5号亚基(chorionic gonadotropin beta polypeptide 5,CGB5)对卵巢癌OVCAR-3细胞血管生成拟态形成的影响。方法采用慢病毒载体转染的方法,在卵巢癌OVCAR-3细胞中分别转入CGB5过表达载体、空载体、CGB5 siRNA及无关序列siRNA。另将未处理的OVCAR-3细胞作为空白对照,高表达CGB5的绒癌细胞Be Wo作为阳性对照。将两种细胞分别注入随机分组的裸鼠右侧腋窝皮下构建皮下移植瘤模型,取肿瘤组织用HE染色及CD34-PAS双染色观察各组中血管生成拟态(vasculogenic mimicry,VM)的形成,并用透射电子显微镜观察各组肿瘤中血管生成拟态的形成。结果 CGB5过表达组的血管生成拟态形成的数量较对照组明显增加,而CGB5干扰组的血管生成拟态形成的数量较对照组明显减少。结论 CGB5能促进卵巢癌OVCAR-3细胞血管生成拟态的形成,这将有望为卵巢癌的治疗提供新的靶标和思路。
Objective To explore the effect of the 5th subunit of β-HCG, chorionic gonadotropin beta polypeptide 5 （CGB5）, on human epithelial ovarian cancer cell line OVCAR-3 in vasculogenic mimicry （VM） formation. Methods The CGB5 overexpressed vector, blank vector, CGB5 siRNA and scramble siRNA were transfected into OVCAR-3 cells. The OVCAR-3 cells without treatment were taken as blank control. The human choriocarcinoma cells BeWo with CGB5 high expression was used as a positive control. These cells were injected subcutaneously into the nude mice. When the tumors grew to an average size of 1-2 cm3, the mice were sacrificed by cervical decapitation. The HE staining, CD34-PAS dual staining and transmission electron microscopy were used to observe the morphology characteristic of VM in xenografts. Results Compared with the control groups, VM channels in xenografts were increased significantly in CGB5- overexpressed OVCAR-3 cells group and reduced significantly in CGB5-knockdown OVCAR-3 cells group. Conclusion CGB5 may promote the formation of VM, it may provide a new way for ovarian cancer therapy.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2016年第9期733-737,共5页
Cancer Research on Prevention and Treatment
基金
国家自然科学基金青年科学基金(30801226)
