摘要
目的建立土荆芥中槲皮素、山奈素、异鼠李素含量的HPLC测定方法。方法采用DIKMA Platisil ODS C_(18)色谱柱(250mm×4.6mm,5μm),流动相为乙腈-0.3%磷酸(38∶62),流速为1.0m L·min^(-1),检测波长为370nm,柱温为30℃。结果槲皮素进样量在0.0559~3.3552μg与峰面积线性关系良好,r=0.9999,平均回收率为104.82%,RSD为1.83%(n=6);山奈素进样量在0.1112~6.6720μg与峰面积线性关系良好,r=0.9999,平均回收率为100.82%,RSD为2.43%(n=6);异鼠李素进样量在0.0128~0.7680μg与峰面积线性关系良好,r=0.9999,平均回收率为99.49%,RSD为2.55%(n=6)。结论本法准确、重复性好,可用于同时测定土荆芥中槲皮素、山奈素、异鼠李素的含量。
OBJECTIVE To develop an HPLC method for determination of quercetin ,kaempferide and isorham-netin in Chenopodii ambrosioidis.METHODS The DIKMA Platisil ODS C18 （250mm ×4.6mm,5μm） column was used.The mobile phase was acetonitrile-0.3%phosphoric acid（38∶62） at a flow rate of 1.0mL· min-1 and at 30℃of column temperature.The detection wavelength was 370nm.RESULTS The linear range for quercetin was 0.0559-3.3552μg,r=0.9999 （n=6）.The average recovery was 104.82% and RSD was 1.83%.The linear range for kaempferide was 0.1112 -6.6720μg, r =0.9999 （ n =6 ） .The average recovery was 100.82% and RSD was 2.43%.The linear range for isorhamnetin was 0.0128-0.7680μg,r=0.9999 （n=6）.The average recovery was 99.49%and RSD was 2.55%.CONCLUSION This method is rapid and accurate ,which can be used to deter-mine the contents of quercetin ,kaempferide aod isorhamnetin in Chenopodii ambrosioidis .
出处
《海峡药学》
2016年第7期65-67,共3页
Strait Pharmaceutical Journal
基金
2013年度福建省中医药科研项目(wzzy201312)
福建省大学生创新项目(201410393066)
关键词
土荆芥
槲皮素
山奈素
异鼠李素
高效液相色谱法
含量测定
Chenopodii ambrosioidis
Quercetin
Kaempferide
Isorhamnetin
HPLC
Quantitative determining
