摘要
目的测定不同产地52份金钱草样品中槲皮素、山柰素、总黄酮及浸出物含量,分析不同产地金钱草的质量差异。方法采集贵州、四川及重庆3个地区不同地点的金钱草,采用HPLC法测定金钱草槲皮素和山柰素含量,Symergy HTX酶标仪测定总黄酮含量。结果52份金钱草样品槲皮素和山柰素总含量在0.1462~2.5170 mg/g,平均含量为0.8726 mg/g,其中四川、贵州、重庆产金钱草槲皮素和山柰素平均含量分别为1.0732、0.7054、0.8651 mg/g。有20份样品含量达到《中华人民共和国药典》标准,达标样品的平均含量为1.4397 mg/g,贵州、四川及重庆采集的样品达标率分别为12.5%、62.5%、38.8%;52份样品总黄酮含量为0.9942~3.8664 mg/g,均符合《中华人民共和国药典》乙醇热浸出物标准。结论川黔渝不同来源地金钱草槲皮素和山柰素总含量差异较大,同一区县采集的金钱草槲皮素和山柰素总含量也存在较大差异,且达标率低;同时野外采集的金钱草样品不同产地的总黄酮存在较大差异。
Objective To determine the contents of quercetin,kaempferol,total flavonoids and extracts in 52 samples of Lysimachiae Herba collected from different origins;To analyze the quality differences of Lysimachiae Herba among different producing areas.Methods The quercetin and kaempferol contents of the Lysimachiae Herba from Guizhou Province,Sichuan Province and Chongqing were determined by HPLC,and the total flavonoids were determined by Symergy HTX microplate reader.Results The total content of quercetin and kaempferol in 52 samples was among 0.1462-2.5170 mg/g,with an average content of 0.8726 mg/g,among which the average content of Sichuan was 1.0732 mg/g,that of Guizhou was 0.7054 mg/g,and that of Chongqing was 0.8651 mg/g.Among them,20 samples reached the standard of the Chinese Pharmacopoeia.The average content of the samples that met the standard was 1.4397 mg/g.The compliance rate of samples collected in Guizhou,Sichuan and Chongqing reached 12.5%,62.5%,and 38.8%respectively.The total flavonoid content of 52 samples was among 0.9942-3.8664 mg/g,and 52 samples were in conformity with the ethanol hot extract standard of the Chinese Pharmacopoeia.Conclusions The total contents of quercetin and kaempferol from different sources in Sichuan,Guizhou and Chongqing are quite different,and the total contents of quercetin and kaempferol collected from the same district and county are also quite different,and the compliance rate is low.There are great differences in total flavonoids in different producing areas and different populations of Lysimachiae Herba samples collected in the field.
作者
练瀚
胡光禄
唐祥友
杨小玉
韦肸
张兴翠
胡开治
Lian Han;Hu Guanglu;Tang Xiangyou;Yang Xiaoyu;Wei Xi;Zhang Xingcui;Hu Kaizhi(School of Agriculture and Biotechnology,Southwest University,Chongqing 400700,China;Chongqing Nanchuan District Hospital of Traditional Chinese Medicine,Chongqing 408435,China;Chongqing Institute of Medicine Plantation,Chongqing 408435,China;College of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550005,China)
出处
《国际中医中药杂志》
2023年第1期67-73,共7页
International Journal of Traditional Chinese Medicine
基金
重庆市技术创新与应用发展专项重点项目(cstc2019jscx-gksbx0010)
国家重点研发计划项目(2021YFD1601005)。
关键词
金钱草
槲皮素
山柰素
总黄酮
高效液相色谱法
中药产地
Lysimachiae Herba
Quercetin
Kaempferol
Total flavonoids
High performance liquid chromatography
Place of production(TCD)
