摘要
目的探讨男性不育患者的精子DNA碎片率(DFI)与精液参数间相关性及精子DFI评价男性生育力方面的应用价值。方法精液标本均取自不育患者,以WHO精液分析标准行精液检测,根据结果分为精液参数正常组、弱精子症组、少精子症组、少弱精子症组。用染色质扩散实验法(SCD)检测精子DFI。结果精液参数正常组与参数异常组(包括弱精子症组、少精子症组和少弱精子症组)间的DFI有明显的差异(P<0.05),不育组间DFI也存在差异(P<0.01)。DFI与精子活动率、前向运动精子率存在明显的负相关性(r=-0.575,r=-0.547,P<0.01),与精子浓度之间无相关性(r=-0.049,P>0.05);精子DFI评价精液参数的ROC曲线下面积(AUC)达0.693,其阈值为19.5%(特异性:89.3%,敏感性:41.8%)。结论随着男性精液质量的下降,精子DFI明显升高,提示精子DNA损伤可能是引起男性不育的重要原因之一。精子DFI>19.5%时,精液质量参数有进一步降低的趋势和风险。精子DFI的检测对评估男性生育能力有重要的临床意义。
Objective To investigate the application value of sperm DNA fragmentation index（DFI） in male patients, and explore the correlation between DFI and semen parameters including motility and concentration. Methods Semen samples were obtained from subfertile men. All semen samples were divided into the normal group and the abnormal groups including asthenozoospermia group,oligozoospermia group and oligoasthenozoospermia group according to World Health Organization guideline. Sperm DNA damage frequency was evaluated by the sperm chromatin dispersion （SCD） test. Results Significant differences were found in sperm DFI between the normal group and the abnormal groups （P〈0.05）, and among the abnormal groups （P〈0.01）. Significant correlations were determined between sperm DFI and sperm viability, motility, rapid forward progressive motility （r=-0.575, r=-0.547, P〈0.01）, but no correlations between sperm DFI and sperm concentration（r=-0.049, P〉0.05） was determined. Area under ROC curve of sperm DFI and sperm parameters reached 0.693, and the cut-off value was 19.5%（sensitivity：41.8%, specificity：89.3%）. Conclusion Sperm DFI increased significantly in parallel with the decrease in sperm quality, indicating that sperm DNA damage might be an important cause of male infertility. Sperm DFI evaluates semen quality, with the cut-off level of 19.5%.The test of sperm DNA fragmentation was helpful to evaluate male reproductive function and the outcome of assisted reproductive technology.
出处
《中国男科学杂志》
CAS
CSCD
2016年第4期19-22,共4页
Chinese Journal of Andrology
基金
云南省科技计划项目联合专项(NO.2010CD201)
关键词
不育
男性
精子
DNA碎片
infertility,male
spermatozoa
DNA fragmentation
