摘要
建立用超高效液相色谱-串联质谱检测器测定食品中的黄曲霉毒素B1、B2、G1、G2的方法。样品中残留的黄曲霉毒素经过乙腈(乙腈∶水=84∶16)提取,取经过4000 r/min离心机离心过的上层清液,经装有反相离子交换吸附剂的多功能净化柱,去除脂肪、蛋白质、色素及碳水化合物等干扰物质,氮吹定容后上机至液相质谱联用仪,根据色谱图出峰时间及与之相应标物的特征离子对作对比,对黄曲霉毒素B1、B2、G1、G2定性,再根据标准系列得出的标准曲线,换算出各黄曲霉毒素的含量。
Determination of aflatoxin B1, B2, G1, G2 in food by ultra performance liquid chromatography tandem mass spectrometry was established. Samples of residual aflatoxin by acetonitrile（acetonitrile∶water=84∶16） extract, take after 4000 r/min centrifugal supernatant fluid and purification by multi-function with reversed-phase ion exchange adsorbent column, remove pigment, protein, fat and carbohydrate substances such as interference. Nitrogen blow after the constant volume computer to liquid mass spectrometry instrument。According to the chromatogram peak time and corresponding with the characteristics of ion to compare on aflatoxin B1, B2, G1, G2.According to the standard series of standard curve figure out the content of aflatoxin.
出处
《广东化工》
CAS
2016年第7期192-194,共3页
Guangdong Chemical Industry
