摘要
目的建立免疫亲和柱净化-HPLC光化学柱后衍生荧光检测法,用于测定植物油中黄曲霉毒素B1、B2、G1、G2。方法样品用乙腈-水（20＋80,体积比）混合液提取,提取液经免疫亲和柱净化,净化液经C18色谱柱分离,用光化学柱后衍生器衍生、荧光检测器进行检测,采用外标法进行定量。结果在优化的条件下,4种黄曲霉毒素能达到很好的分离效果,黄曲霉毒素B1、G1在0.2~20.0 ng/ml范围内线性关系良好,检出限为0.04μg/kg;黄曲霉毒素B2、G2在0.1~10.0 ng/ml范围内线性关系良好,检出限为0.02μg/kg,4种黄曲霉毒素的回收率为82.5%~95.3%,RSD为2.4%~5.9%。结论该方法快速、简单、准确、灵敏、重现性好,是测定植物油中的黄曲霉毒素B1、B2、G1、G2的可靠方法。
Objective To establish a method for the determination of aflatoxins B1、B2、G1、G2in vegetable oils by cleaning up immunoaffinity column and high performance liquid chromatography（ HPLC） of fluorescent light with post- column photochemical derivatization. Methods The samples were extracted with acetonitrile- water solution（ 20 ＋ 80,V ＋ V）,the extractions were cleaning up by immunoaffinity column,the purified liquid were separated by using C18 chromatographic column,derived with post- column photochemical derivatization,and examined by fluorescence detector and quantified using external standard method. Results Under the condition of optimization,the four kinds of aflatoxin were separated completely,aflatoxin B1 and G1had a good linear relationship between 0. 2 ng / ml and 20. 0 ng / ml with the detection limit0. 04 μg / kg,and aflatoxin B2 and G2between 0. 1 ng / ml and 10. 0 ng / ml with the detection limit 0. 02 μg / kg,the recovery rates of four kinds of aflatoxin were 82. 5%- 95. 3%,and RSD were 2. 4%- 5. 9%. Conclusion The method is reliable for determination of aflatoxins B1、B2、G1、G2with advantages of rapidness,simpleness,accuracy,sensitiveness and good reproducibility.
出处
《中国卫生工程学》
CAS
2016年第4期383-384,387,共3页
Chinese Journal of Public Health Engineering
关键词
免疫亲和柱
光化学柱后衍生
高效液相色谱
黄曲霉毒素
植物油
Immunoaffinity column
Post-column photochemical derivatization.
High performance liquid chromatography（HPLC）
Aflatoxins
Vegetable oil
