摘要
[目的]建立超高效液相色谱-串联质谱法（UPLC-MS/MS）定量测定肉类中的克伦特罗、莱克多巴胺、沙丁胺醇及特布他林4种β-受体激动剂的方法。[方法]样品经β-葡萄糖醛苷酶/芳基硫酸酯酶酶解,再经SLW固相萃取柱净化后,采用UPLC-MS/MS方法测定4种β-受体激动剂。[结果]4种β-受体激动剂在0.1-20μg/L测定的范围内线性关系良好（r〉0.999 6）,方法的检出限为0.03-0.10μg/kg（S/N=3）。在加标水平为2、5、10μg/kg时,空白样品的加标回收率为70.3%-97.1%,相对标准偏差为0.25%-4.50%。[结论]该方法分析时间短、选择性好、灵敏度高,适用于肉类中的β-受体激动剂的检测。
[ Objective ] To establish a method for the determination of four β-agonists including clenbuterol, ractopamine, salbutamol, and terbutaline in meat products induced by ultra performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）. [ Methods ] The test samples were enzymatically hydrolyzed with β-glucuronidase/arylsulfatase and purified by SLW solid phase extraction column. The residues were detected for four β-agonists by UPLC-MS/MS. [ Results ] The proposed method showed a good linearity over the range of 0.1-20 μg/L for the four β-agonists with r 〉 0.999 6 and the limit of detection （LOD） of 0.03-0.10 μg/kg （S/N=3）. The average recoveries were 70.3%-97.1% and the relative standard deviations were 0.25%-4.5% when the samples were spiked at levels of 2, 5, and 10 μg/kg. [ Conclusion ] This highly effective, selective, and sensitive method is suitable for detecting β-agonists in meat products.
出处
《环境与职业医学》
CAS
CSCD
北大核心
2016年第2期193-196,共4页
Journal of Environmental and Occupational Medicine
关键词
超高效液相色-串联质谱
固相萃取
肉类
β-受体激动剂
酶解
ultra performance liquid chromatography-tandem mass spectrometry
solid phase extraction
meat
β-agonist
enzymatic hydrolyzation
