摘要
建立一种同时测定猪肉中3种β-受体激动剂残留量的高效液相色谱-电喷雾串联质谱(HPLC-ESI-MS/MS)确证分析方法。样品经β-葡萄糖醛酸酶/芳基硫酸酯酶酶解、乙酸铵缓冲液提取和MCX固相萃取柱净化,采用Agilent ZorbaxSB-C18(2.1mm×150mm,3.5μm)色谱柱,0.1%的甲酸水溶液、甲醇和乙腈作为流动相进行洗脱,高效液相色谱分离,电喷雾离子源电离,正离子多反应监测模式进行检测,内标法定量。3种药物在0.05～1μg/kg浓度范围内线性良好,相关系数r均大于0.999,0.05、0.1、0.5μg/kg3个浓度水平的添加回收率在89.7%～106.7%之间,相对标准偏差为2.4%～8.6%,3种药物的定量限均为0.05μg/kg。方法适用于猪肉中β-受体激动剂残留的确证分析。
A confirmative method was developed by high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) to detect 3 beta-agonist residues in pork simultaneously,which included clenbuterol,ractopamine,salbutamol.In the method,The analytes were extracted with NH4Ac solution from homogenized samples and hydrolysised with beta-glucuronidase overnight at 37 ℃.Then the extract was filtered and the filtrate was cleaned by oasis MCX solid phase extraction cartrid...
出处
《分析试验室》
CAS
CSCD
北大核心
2010年第4期92-98,共7页
Chinese Journal of Analysis Laboratory
关键词
高效液相色谱-电喷雾串联质谱
β-受体激动剂
猪肉
High performance liquid chromatography-electrospray ionization tandem mass spectrometry
Beta-agonist
Pork
