摘要
以鹿茸干细胞为研究对象,分别采用甲基化敏感性扩增多态性(MSAP)和荧光标记甲基化敏感性扩增多态性(F-MSAP)方法对鹿茸干细胞进行全基因组DNA甲基化检测。结果表明:MSAP方法共检测到387个位点,F-MSAP方法共检测出1 524个位点。2种方法所得结果中均发现Ⅰ型条带数最多,Ⅲ型条带数最少。与MSAP方法比较,F-MSAP方法在数据分析和实际操作过程中具有安全、高效、高通量、自动化等特点,更适用于检测鹿茸干细胞基因组DNA甲基化。
The methylation-sensitive amplification polymorphism（ MSAP） and the fluorescence-labeled methylation-sensitive amplification polymorphism（ F-MSAP） are both modified AFLP（ amplified fragment length polymorphism） techniques to investigate cytosine methylation in genomes. In FMSAP system,selective amplification is fluorescently labeled and selective products could be detected by DNA sequencer. In the present study,MSAP and F-MSAP methods were used to analyze DNA methylation in antler stem cells. In MSAP and F-MSAP systems,a total of 387 and 1 524 fragments were detected,respectively,and type I fragments are the most frequent and type Ⅲ fragments are the least frequent. The results show that compared to MSAP method,F-MSAP method had the main advantages of safety,high efficiency,high sensitivity and automation in the process of data analysis and operation. Overall,the present study reveals that the F-MSAP method was more suitable than MSAP method for detecting cytosine methylation in antler stem cells as well as deer.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2016年第1期97-101,共5页
Journal of Jilin Agricultural University
基金
国家自然科学基金青年基金项目(31402059)
