摘要
KDM3家族成员中KDM3A、KDM3B能够特异性去除H3K9me1与H3K9me2的甲基化修饰,而JMJD1C作为KDM3家族成员之一,与KDM3A、KDM3B即JMJD1A、JMJD1B含有相似的Jmj C结构域,但JMJD1C是否也具有对H3K9的催化作用或活性一直不明确。本研究利用RNAi方法抑制JMJD1C的mRNA表达水平,随后通过免疫荧光方法检测H3K9me1与H3K9me2的甲基化程度。结果表明,3T3-L1细胞和卵丘细胞中均存在JMJD1C的表达,并且存在H3K9me1与H3K9me2的甲基化修饰。通过siRNA对牛卵丘细胞中JMJD1C mRNA的表达进行成功抑制后,转染组JMJD1C的表达量明显下降,但免疫荧光结果显示H3K9me1、H3K9me2表达量无明显变化,即JMJD1C在mRNA水平上对去除H3K9me1与H3K9me2的甲基化修饰并无明显作用。
KDM3A and KDM3 B which are members of KDM3 subfamily can remove specifically H3K9me1 and H3K9me2 methylation modification. As a member of KDM3 subfamily,JMJD1 C contains similar Jmj C domain to KDM3 A and KDM3 B which are called JMJD1 A and JMJD1 B,separately. However the activity of JMJD1 C towards H3K9 methylation is still unknown. In this study,we inhibited mRNA expressing level of JMJD1 C using the method of RNAi and detected H3K9me1 and H3K9me2 methylation level by immunofluorescence technology. The results show that JMJD1 C was expressed in both 3T3-L1 cells and bovine cumulus cells. At the same time,the modification of H3K9me1 and H3K9me2 methylation existed in both cells. Comparing to the control group,there was significantly decline of JMJD1 C expressing level in transfected groups after the mRNA expressing of JMJD1 C was inhibited successfully in bovine cumulus cells through RNAi. And the immunofluorescence results show that there was no significant change in the expressing of H3K9me1 and H3K9me2. That is to say JMJD1 C has no significant effect on removing H3K9me1 and H3K9me2 methylation at the mRNA level.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2015年第2期325-329,共5页
Chinese Journal of Veterinary Science
基金
国家科技支撑计划资助项目(2011BAD19B04)
吉林省自然科学基金资助项目(201215010)
吉林省科技厅重点科技攻关资助项目(20140204077NY)
现代农业产业技术体系建设专项资金资助项目(CARS-38)
