摘要
DNA甲基化在植物的生长发育中具有重要的调控作用,它是植物正常生长发育所必需的。采用甲基化敏感扩增多态性(Methylation sensitive amplification polymorphism,MSAP)技术,以羽衣甘蓝自交不亲和系9#种子为研究对象,从表观遗传学的角度,对其生长发育过程以及高压静电场处理条件下基因组DNA甲基化水平及模式变化情况进行研究,探讨自交不亲和系种子退化现象与表观遗传规律的相关性。结果表明,自交不亲和系9#种子经高压静电场处理后,同时发生甲基化与去甲基化事件,以去甲基化为主。其中全甲基化水平略有升高,半甲基化水平下降明显,总甲基化水平下降;通过记录和计算种子在萌发过程中的各项指标,结果表明,高压静电场可促进9#种子的萌发和幼苗的生长发育,改善种子品质,表明高压静电场处理是恢复或延迟羽衣甘蓝自交不亲和系退化种子生长势,保持自交后代品质的有效手段之一。
DNA methylation plays an important role in regulating plant growth,and it is necessary for plant normal growth and development. In this experiment,methylation sensitive amplification polymorphism( MSAP) was used to study the status and patterns of the DNA methylation at different periods in seed germination of self-incompatible line 9#and the change of DNA methylation status in 9#after high-voltage electric field. The correlation was discussed between seed degradation phenomena of self-incompatible line and epigenetic one. The results were shown as follows: After high-voltage electric field treatment,both methylation and demethylation occurred; and demethylation seemed to be predominant,the proportion of full-methylation increased slightly and semi-methylation declined clearly and brought about the result of total methylation declined; the physiological indexes of 9#in seed germination,which showed high-voltage electric field,could promote seed germination and seeding growth and improve the quality of seeds. High-voltage electric field was an effective means to delay and restore growth vigor,and to maintain the quality of self-crossed progeny.
出处
《华北农学报》
CSCD
北大核心
2015年第S1期8-18,共11页
Acta Agriculturae Boreali-Sinica
基金
中央高校基本科研业务费专项资金项目(2013BAQ01)
哈尔滨市科技创新人才研究专项资金项目(青年科技创新人才)(2013RFQXJ036)
关键词
羽衣甘蓝
自交不亲和性
DNA甲基化
高压静电场
Brassica oleracea var.acephala
Self-incompatibility
DNA methylation
High-voltage electric field
