摘要
目的了解5-HTTLPR基因多态性与学龄双生子儿童焦虑抑郁的关系。方法选取内蒙古呼和浩特市和包头市8~12岁147对双生子儿童（同卵双生子47对;异卵双生子100对）为调查对象,运用Achenbach儿童行为量表（CBCL）测算儿童焦虑抑郁因子得分;采集所有儿童口腔上皮细胞并提取DNA,运用聚合酶链式反应（PCR）方法对5-HTTLPR基因进行分型;利用广义估计方程（GEE）分析5-HTTLPR基因多态性及家庭环境因素对学龄双生子儿童焦虑抑郁的影响。结果 LS和SS基因型儿童的焦虑抑郁因子得分高于LL基因型儿童（χ2=3.938,P〈0.05）;5-HTTLPR基因型与家庭亲密度（χ2=6.129,P〈0.05）和家庭教养方式（χ2=7.665,P〈0.05）的交互作用对儿童焦虑抑郁因子得分影响显著。结论 5-HTTLPR基因型与学龄双生子儿童焦虑抑郁因子得分显著相关;在高亲密度和专制型教养方式的家庭环境中,S等位基因可能增加双生子儿童焦虑抑郁的可能性。
Objective To investigate the association between anxiety-depression and 5-HTTLPR gene polymorphism in school-aged twins. Methods A total of 147 pairs of twins（47 pairs of monozygotic twins, 100 pairs of dizygotic twins） aged 8-12 years from Baotou and Hohhot were selected as respondents. The Achenbach Child Behavior Checklist（CBCL） was used to calculate the scores of anxiety-depression factors in school-aged twins. The DNA was extracted from oral epithelial cells, and polymerase chain reaction was applied for 5-HTTLPR genotyping. The generalized estimating equation（GEE） was used to analyze the effect of 5-HTTLPR polymorphism and family environment on anxiety-depression in school-aged twins. Results The children with LS and SS genotypes had significantly higher scores of anxiety-depression factors than those with LL genotype（χ2=3.938, P〈0.05）. The interaction of 5-HTTLPR genotype with family cohesion and family rearing patterns had a significant impact on the scores of anxiety-depression factors in twins（χ2=6.129 and 7.665, both P〈0.05）. Conclusions 5-HTTLPR genotype is significantly correlated with the scores of anxiety-depression factors in school-aged twins. In the family with high cohesion and an autocratic family rearing pattern, S allele may increase the possibility of anxiety-depression in twin children.
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2016年第1期61-66,共6页
Chinese Journal of Contemporary Pediatrics
基金
国家自然科学基金(31260265
30960167)
