摘要
从油田采出水中富集培养石油烃降解菌,利用^(13) C标记的甲苯作为碳源和变性梯度凝胶电泳技术,从中鉴定和考察能够降解甲苯的细菌群落。结果表明:从采出水中富集培养的复合菌群经过500d的厌氧降解,对石油烃降解率达到36.4%,甲烷产量为201.2μmol;以^(13) C标记的甲苯为碳源,经过60d的培养,复合菌群对其降解率达到67%,12 C标记的甲苯降解率为69%,两种碳源标记甲苯的降解率差异不显著;将离心中的重带^(13) C-DNA片段回收测序,鉴别出3种芳烃降解菌。
The degradation bacteria of petroleum hydrocarbon were enriched and cultured from the oilfield water. Using 13 C-toluene as carbon source and denaturing gradient gel electrophoresis ( DGGE) technology to identify and investigate the bac-terial community which could degrade the toluene. The results show that after 500 days anaerobic degradation the petroleum hydrocarbon degradation rate is 36. 4% by complex bacterium, and the methane yield is 201. 2μmol. It is also found that af-ter 60 days culture the carbon source13 C-toluene degradation rate is 67% by the complex bacterium. Meanwhile, after 60 days culture the 12 C-toluene degradation rate is 69%, and two kinds of toluene degradation rate are not significantly different. 13 C-DNA is recovered and identified for three types of aromatic hydrocarbon degrading bacteria.
出处
《中国石油大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2015年第6期57-62,共6页
Journal of China University of Petroleum(Edition of Natural Science)
基金
中国石油天然气集团公司科学研究与技术开发项目(2008D-4704-2)
