摘要
研究通过逐渐提高培养液的盐浓度从含石油烃的钻井泥浆中驯化获得四株耐盐的石油烃降解菌,筛选出1株对原油降解效率高的优势菌株SW-1。经16S rRNA基因序列分析确定其系统发育地位,采用单因素实验研究环境因素对该菌原油降解率的影响,研究其对典型石油烃类物质的降解能力及降解特性。结果表明:石油烃降解菌耐受的盐度为9%;盐度为0时,菌株SW-1的原油降解率为51.49%;16S rRNA基因序列比对结果显示,该菌株与Bacillus licheniformis MGB70112.1核苷酸序列相似性为100%;pH值为9,温度为30℃降解效果最佳;在9%盐浓度,最佳条件下培养7 d,SW-1菌株对原油的降解率为33.10%,对菲的降解率为46.53%;GC-MS分析结果表明,菌株SW-1可以降解链长为C19~C28的烷烃,C19~C28烷烃的平均降解率达到18.48%。
In this study,four salt tolerant strains of petroleum hydrocarbon degrading bacteria were domesticated from drilling mud containing petroleum hydrocarbon by gradually increasing the salt concentration of the culture solution,and a dominant strain SW-1 with high crude oil degradation efficiency was selected.The phylogenetic status was determined by 16S rRNA gene sequence analysis,a single factor experiment was used to study the influence of environmental factors on the crude oil degradation rate of the bacteria,the degradation ability and characteristics of typical petroleum hydrocarbons were studied.The results showed that the tolerance salinity of petroleum degrading bacteria was 9%;When the salinity was 0,the crude oil degradation rate of strain SW-1 was 51.49%;16S rRNA gene sequence alignment results showed that,the nucleotide sequence similarity between the strain and Bacillus licheniformis MGB70112.1 was 100%;The pH was 9 and the temperature was 300℃,the degradation effect was the best;At 9%salt concentration,incubate for 7 days under optimal conditions,the degradation rate of crude oil by SW-1 strain was 33.10%,the degradation rate of phenanthrene was 46.53%;The results of GC-MS analysis show that strain SW-1 can degrade alkane with a chain length of C19-C28,with the average degradation rate up to 18.48%.
作者
龚汉意
邓春萍
柯强
邱肃爽
杜国勇
Gong Hanyi;Deng Chunping;Ke Qiang;Qiu Sushuang;Du Guoyong(College of chemistry and chemical engineering,Southwest Petroleum University,Chengdu 610500,China)
出处
《山东化工》
CAS
2021年第4期39-42,共4页
Shandong Chemical Industry
基金
四川省科技计划项目(2019YJ0514)
西南石油大学科研“启航计划”项目(2018QHZ018)。
关键词
石油烃
耐盐
生物降解
降解特性
petroleum hydrocarbon
salt tolerance
biodegradation
degradation characteristics
