摘要
通过构建16S/18S r DNA基因文库,分析自由表面流人工湿地污水处理系统春季空气细菌和空气真菌群落结构特征.结果表明,空气细菌分布在变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、浮霉菌门(Planctomycetes)、蓝藻门(Cyanophyta)、绿弯菌门(Chloroflexi)、拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes),主要为β-变形菌纲(71.04%)、γ-变形菌纲(12.03%)、α-变形菌纲(3.83%)、蓝藻纲(4.38%)、芽孢杆菌纲(3.28%)和鞘脂杆菌纲(2.19%),优势菌属是马赛菌属(Massilia 66.66%)、假单胞菌属(Pseudomonas 4.37%)、蓝丝细菌属(Cyanothece 3.83%)和沙雷氏菌属(Serratia 3.28%).空气真菌主要类群为座囊菌纲(Dothideomycetes 61.18%),其次是接合菌纲(Zygomycetes16.47%)、盘菌纲(Discomycetes 14.12%),优势菌属是核腔菌属(Pyrenophora 48.31%)、被孢霉属(Mortierella 15.7%)、缘刺盘菌属(Cheilymenia12.4%)、Boothiomyces(4.5%).人工湿地空气微生物中未检测出大肠杆菌(Escherichia coli)、沙门氏菌(Salmonella spp.)和产气荚膜梭菌(Clostridium perfringens),但存在粘质沙雷氏菌(S.marcescens)、恶臭假单胞菌(P.putida)、表皮葡萄球菌(Staphylococcus epidermidis)等致病菌或条件致病菌.
Airborne microbes are major biological components of the ecosystem and have important ecological functions. They affect human health, cause air pollution, and are key indicators of air quality. To determine the community structure of airborne microbes in a surface-flow wetland system constructed for sewage treatment in spring, the airborne bacteria and airborne fungi were analyzed by a 16S/18S rDNA gene clone library method. Phylogenetic analysis of airborne bacteria in the airborne microbes sample showed that there were seven groups： Proteobacteria, Actinobacteria, Planctomycetes, Cyanophyta, Chloroflexi, Bacteroidetes and Firmicutes. The main types of airborne bacteria were Betaproteobacteria （71.04%）, Gammaproteobacteria （12.03%）, Alphaproteobacteria （3.83%）, Cyanophyceae （4.38%）, Bacilli （3.28%） and Sphingobacteriales （2.19%）, while the dominant genera of airborne bacteria included Massilia （66.66%）, Pseudomonas （4.37%）, Cyanothece （3.83%） and Serratia （3.28%）. Phylogenetic analysis of airborne fungi in the airborne microbes sample showed that the main types were Dothideomycetes （61.18%）, Zygomycetes （16.47%） and Discomycetes （14.12%）. The dominant genera of airborne fungi included Pyrenophora （48.31%）, Mortierella （15.7%）, Cheilymenia （12.4%） and Boothiomyces （4.5%）. Escherichia coli, Salmonella spp. and Clostridium perfringens were not detected in the sample of airborne microbe while other pathogens or conditioned pathogens, such as S. marcescens, p. putida, and Staphylococcus epidermidis were detected.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2014年第12期3164-3174,共11页
China Environmental Science
基金
国家自然科学基金资助项目(31170509)
关键词
人工湿地
空气细菌
空气真菌
群落结构
基因文库
constructed wetland
airborne bacteria
airborne fungi
community structure
genomic library
