摘要
为了解低温(15.0～16.5℃)稳定运行的厌氧氨氧化生物滤池微生物特征,利用扫描电镜(SEM)、变性梯度凝胶电泳技术(DGGE)和克隆测序等方法,对低温稳定运行的两个上流式厌氧氨氧化生物滤池进行微生物群落结构分析.SEM结果显示,陶粒填料反应器(B1)内丝状菌较多,球形细菌分布密度较低,而火山岩填料反应器(B2)没有发现丝状菌,球形细菌分布密集.DGGE结果表明,B1与B2反应器微生物种类有所差别,种群相似性为68.1%,B2反应器内细菌丰度更高,但两反应器内厌氧氨氧化细菌种类相同.通过细菌及ANAMMOX菌16S rRNA克隆测序,鉴定反应器内厌氧氨氧化细菌为Candidatus Kueneniastuttgartiensis.采用火山岩填料生物滤池反应器形式,并富集Candidatus Kuenenia stuttgartiensis,有助于厌氧氨氧化工艺在较低温度(15.0～16.5℃)条件下稳定运行和推广应用.
To characterize the microbial community in anaerobic ammonia oxidation（ANAMMOX） biofilter and to apply this technology,techniques of scanning electron microscopy（SEM）,denaturing gradient gel electrophoresis（DGGE）,cloning and sequencing were utilized to study the community structures in two up-flow ANAMMOX biofilters,which were operated stably at low temperature（15.0-16.5 ℃）.The results of SEM showed that the filamentous bacteria were just found within ceramic packing B1 biofilter.Spherical bacteria had more densely distribution in volcanic packing B2 biofilter than that in B1 biofilter.Bacterial DGGE results showed that there were some differences between B1 and B2 microbial species and the population similarity was 68.1%,and B2 reactor had higher bacteria richness.However,the type of anammox bacteria existence in two reactors was the same.The sequences of bacteria and ANAMMOX 16S rRNA identification revealed that Candidatus Kuenenia stuttgartiensis occurred in two ANAMMOX bioreactors.It was helpful to apply ANAMMOX technology at low temperature（15.0-16.5 ℃） through Candidatus Kuenenia stuttgartiensis enrichment,with the form of biofilter reactor filled with volcanic rocks.
出处
《哈尔滨工业大学学报》
EI
CAS
CSCD
北大核心
2012年第6期6-10,共5页
Journal of Harbin Institute of Technology
基金
教育部跨世纪优秀人才支持计划(NCET-10-0008)
城市水资源与水环境国这有重点实验室开放基金(QAK201005)
国家重大科技专项水专项(2012ZX07202-005)
关键词
厌氧氨氧化
变性梯度凝胶电泳
生物滤池
低温
anaerobic ammonium oxidation
denaturing gradient gel electrophoresis（DGGE）
biofilter
low temperature
