摘要
针对传统生物工艺处理RO浓液耐盐性差、处理效率低等问题,本研究利用耐盐菌强化厌氧/好氧(A/O)活性污泥耦合催化臭氧氧化工艺以提升有机物和总氮的去除,对比活性污泥、耐盐菌剂、活性污泥+耐盐菌剂的3种体系对RO浓液中污染物去除效果、处理前后有机物成分变化以及微生物特性差异.结果表明,较单独厌氧活性污泥和耐盐菌体系,厌氧强化体系有机氮的转化率分别提高了42%和37%;较单独好氧活性污泥和耐盐菌体系,好氧强化体系总氮去除率提升了37%和77%.气质联用分析显示,原水有机物经生物和臭氧氧化处理后促使一些不饱和键断裂,提高了难降解有机物的降解.处理后,水质指标可达《生活垃圾填埋场污染控制标准》表2标准.高通量测序结果表明,Flavobacterium可能在厌氧强化体系高盐条件下的氨化过程中发挥关键作用;而Truepera、Paracoccus是好氧强化体系高盐条件下总氮高效去除的关键功能菌属.功能基因预测结果显示,氨化基因npr是Flavobacterium菌属的关键基因,进一步证实测序结果推测;好氧强化体系中nap、nar、nir、nor反硝化基因的相对丰度均高于单独体系,揭示了强化体系总氮高效去除的机理.
Aiming at the problems of poor salt tolerance and low treatment efficiency of RO concentrated solution treated by traditional biological treatment process,this study used salt-tolerant bacteria to strengthen the anaerobic/aerobic(A/O)activated sludge coupled catalytic ozonation process to improve the removal of organic matter and total nitrogen.The removal efficiency of pollutants in RO concentrate,the change of organic matter composition before and after treatment,and the differences in microbial characteristics of the three systems of activated sludge,salt-tolerant bacteria and activated sludge/salt-tolerant bacteria were compared.The results showed that the conversion rate of organic nitrogen in anaerobic enhanced system was increased by 42%and 37%,respectively,compared with single anaerobic activated sludge and salt-tolerant bacteria system,and compared with the single aerobic activated sludge and salt-tolerant bacteria system,the total nitrogen removal rate of aerobic enhanced system increased by 37%and 77%.GC-MS analysis showed that some unsaturated bonds were broken after biological and ozone oxidation treatment of raw water organic matter,which improved the degradation of refractory organics.After treatment,the water quality index can reach the Table 2 standard of“Standard for Pollution Control of Domestic Waste Landfill”.High-throughput sequencing results showed that Flavobacterium may play a key role in the ammoniation process under high salt conditions in the anaerobic enhanced system;while Truepera and Paracoccus are the key functional bacteria for efficient removal of total nitrogen under high salinity conditions in aerobic enhanced system.The functional gene prediction results showed that the ammoniation gene npr was the key gene of Flavobacterium,which further confirmed the sequencing results.The relative abundances of nap,nar,nir and nor denitrification genes in the aerobic strengthening system were higher than those in the single system,revealing the mechanism of efficient removal of total nitrogen in the strengthening system.
作者
袁春波
朱宇楠
张楚
刘伟
楼紫阳
张千
YUAN Chunbo;ZHU Yunan;ZHANG Chu;LIU Wei;LOU Ziyang;ZHANG Qian(School of Chemical Engineering,Chongqing University of Technology,Chongqing,400054,China;Shanghai Jiao Tong University Chongqing Research Institute,Chongqing,401147,China;School of Environmental Science and Engineering,Shanghai Jiao Tong University,Shanghai,200240,China)
出处
《环境化学》
CAS
CSCD
北大核心
2024年第1期323-336,共14页
Environmental Chemistry
基金
国家自然科学基金(51908099)
重庆市教委科学技术研究项目(KJQN202001114)资助。
关键词
RO浓液
耐盐菌
生物强化
微生物群落特性
reverse osmosis concentrate
salt-resistant bacteria
biological enhancement
microbial community characteristics
