摘要
目的 :观察缺血预处理对肝脏再灌注损伤的影响。方法 :通过构建正常大鼠和肝硬化大鼠肝脏 70 %的原位热缺血再灌注损伤模型 ,比较缺血预处理和无预处理组及间歇阻断肝门法对再灌注损伤的影响 ;以肝功酶、能量代谢和过氧化损伤等生物化学指标、组织学病理和细胞超微结构的形态学指标 ,观察不同预处理条件对结果的影响。结果 :各项指标显示 ,正常大鼠中 ,缺血预处理与无预处理组相比 ,可显著减轻再灌注损伤 (P <0 .0 5 ) ,与间歇性阻断肝门组相比也有显著性意义 (P <0 .0 5 ) ;在各个缺血预处理组内 ,缺血预处理 5min(IPC5min)组较IPC10min组和IPC5min× 2 组效果好 (P <0 .0 5 ) ,证实缺血预处理对肝硬化大鼠肝脏再灌注损伤有保护作用。结论 :缺血预处理可减轻肝脏再灌注损伤 ,并优于间歇性阻断肝门法 ,是一种应用方便、效果较好。
Objective:To evaluate the influnce of ischemic preconditioning(IPC) on liver ischemic reperfusion injury(IRPI).Methods:70% hepatic ischemia-reperfusion model were performed in normal and cirrotic Wistar rats.Ischemic preconditioning , non-ischemic preconditioning and intermittent groups were compared on IRPI, by serum liver enzymes,oxidative injury,histological and ultra strcture.Results: For both normal and cirrotic liver, preconditioning attenuated IRPI, compared with non-IPC and intermittent group.In normal rats IPC 5minshowed the best results compared with IPC 10minand IPC 5min×2Conclusion:This study proves that preconditioning,as a endogernous anti-reperfusion injury mechanism,can attenuates hepatic reperfusion injury.As a new hepatic occlusion method,preconditioning will be helpful to liver surgery and liver transplantation.
出处
《中国现代普通外科进展》
CAS
2002年第2期87-91,共5页
Chinese Journal of Current Advances in General Surgery
