摘要
建立了玉米幼胚高效再生系统。经研究发现 ,苏玉 1号、农大 3 13 8、农大 10 8的幼胚培养在含有 2 ,4 D ( 2 .5mg/L)的IM培养基上后 ,大多数幼胚能愈伤化并增大 ,形成基部相连、上部分开的微芽结构 ;微芽结构在转移到BM培养基上后 ,形成小植株 ;进一步转移到RM培养基上 ,它们长根并形成完整植株。玉米幼胚高效再生植株与下列因素有关 :玉米基因型、幼胚大小、幼胚长芽至分化时间、6 BA、IBA、Gelrite。不同品种玉米再生能力有显著差异 ,幼胚大小在 1～ 2mm之间再生能力强 ,幼胚长芽至分化时间 4～ 6d最好。激素 6 BA浓度在 0 .5～ 0 .6mg/L之间有利于微芽形成小植株 ,IBA浓度在 0 .6～ 1.0mg/L促进生根。Gelrite可代替琼脂粉用于玉米生根。
In this study, we built the regeneration system of maize immature embryo. Immature embryos of maize Suyu No.1, Nongda 3138, Nongda 108 can become large and formed micro bud construction on MS medium supplemented with 2,4 D 2.5 mg/L(Table 1,Plate I 2). After being transferred to BM medium, micro bud formed plantlet, which came intact plant with roots on RM medium(Plate I 5). The regeneration of maize immature embryo is related to the following factors: maize genetype, length of immature embryo, the time from budding to differentiation, the presence and concentration of 6 BA, IBA and Gelrite. Different maize varieties have different regeneration capacities, the best combination is that length of immature embryo is 1-2 mm, the time from buding to differentiation is 4-6 days, 6 BA is 0.5-0.6 mg/L, IBA is 0.6-1.0 mg/L for root growth. Gelrite could replace agar for maize rooting.
基金
中国科学院上海植物生理研究所国家重点实验室资助(项目批准号 10 4KF990 5 )
