摘要
目的 观察肿瘤转移抑制基因 nm2 3的表达产物二磷酸核苷激酶 (nucleoside diphosphatkinase,NDPK)在裸小鼠玻璃体腔视网膜母细胞瘤 (retinoblastoma,RB)移植瘤中的表达状态 ,探讨 nm2 3基因的表达产物 NDPK(nm2 3/ NDPK)与 RB的发生发展及浸润生长的相关性。 方法 对 2 0例裸小鼠玻璃体 RB移植瘤进行 nm2 3/ NDPK表达的免疫组织化学染色 ,以正常视网膜组织标本作对照 ,比较 RB移植瘤眼内生长期 ( ～ 级 )及蔓延、扩展浸润期 ( ～ 级 ) nm2 3/ NDPK的表达情况。 结果 正常视网膜组织 nm2 3蛋白表达阴性。 2 0例 RB移植瘤 nm2 3/ NDPK表达阳性 ,阳性细胞数为 48.73± 2 .37,阳性表达率为 10 0 % ;眼内生长期 ( ～ 级 )与蔓延、扩展浸润期 ( ～ 级 ) nm 2 3/ NDPK阳性表达细胞数差异无显著性的意义 (P>0 .0 5 )。 结论 nm 2 3基因不具有抑制 RB移植瘤蔓延浸润的功能 ,而是在其发生发展过程中起一定作用。
Purpose To investigate nucleoside diphosphate kinase (NDPK ) expression of tumor metastasis suppressor gene nm23 in heterotransplanted model of retinoblastoma(RB) in nude mice,and analyse the correlation between the expression of nm23 gene and the formation and progression of heterotransplanted RB. Methods SP immunohistochemical method was used to detect the expression of nm23 gene product NDPK in 20 tumors of heterotransplanted RB model and normal retinal tissue. Results The negative staining of nm23/ NDPK was found in normal retinal tissue , whereas 100% expression rate in RB tumors with positive number of 48.73±2.37. No statistical significance of the expression of nm23/ NDPK was observed between the intraocular growth phase (I～Ⅲ grade) and invasive phase (Ⅳ～Ⅴ grade) in heterotransplantedRB tumors. Conclusion The function of nm23 gene as a tumor metastasis suppressor in heterotransplanted RB tumors was less prominent ,but it may play a role in carcinogenesis and progrssion of RB and may predict poor prognosis.
出处
《中华眼底病杂志》
CAS
CSCD
2001年第1期47-49,共3页
Chinese Journal of Ocular Fundus Diseases
基金
国家自然科学基金资助项目! (39570 751 )
中国医学科学院科学基金资助项目
CMB科学基金资助项目! (950 9)
关键词
视网膜母细胞瘤
动物模型
基因表达
NM23基因
Retinoblastoma
Genes, suppressor, tumor
Disease models,animal
Gene expression
nm23 Gene
