摘要
【目的】为了设计和寻找DNA的特异性识别剂和断裂剂,合成了2-氨甲基苯并咪唑铜(Ⅱ)配合物:[Cu(AMB)2Cl]Cl·4H2O(配合物1)和[Cu(AMB)(phen)Cl]Cl·2H2O(配合物2)(AMB=2-氨甲基苯并咪唑,phen=1,10-邻菲咯啉).【方法】通过元素分析、IR、UV和摩尔电导率对配合物进行了表征.用二倍稀释法测试了配合物对大肠埃希菌Escherichia coil(G-)、沙门杆菌Salmonella typhi(G-)、金黄色葡萄球菌Staphylococcus aureus(G+)和枯草芽孢杆菌Bacillus subtilis(G+)的最小抑菌浓度(MIC).采用电子吸收光谱、荧光光谱、相对黏度及琼脂糖凝胶电泳法测试了2个配合物与ct-DNA的结合作用.【结果和结论】配合物1以非插入方式、配合物2以插入方式与ct-DNA作用;在VC存在下,2个配合物均通过·OH氧化机理切割pBR322 DNA.2个配合物结合ct-DNA和切割pBR322 DNA的能力强弱均为:配合物2>配合物1.
[Objective]In order to design and find specific recognition and cleavage agent of DNA , two copper（Ⅱ） complexes,[Cu（AMB）2Cl]Cl· 4H2O（complex 1）and[Cu（AMB）（phen）Cl]Cl· 2H2O （complex 2）（AMB =2-aminomethylbenzimidazole, phen =1,10-phenanthroline）, were synthesized.[Method]The two complexes were characterized by elemental analysis , IR, UV and molar conductivity . The complex 1 and complex 2 were assayed against gram-positive bacteria （ Staphylococcus aureus and Ba-cillus subtilis） and gram-negative bacteria （ Escherichia coil and Salmonella typhi） by a double dilution method, and the interaction with ct-DNA was investigated by electronic absorption spectroscopy , fluores-cence spectroscopy , viscosity measurements and agarose gel electrophoresis .[Result and conclusion]The complex 2 could interact with DNA by non-intercalation, while complex 1 by non-intercalation, and they cleave pBR322 DNA assisted by vitamin C involving the hydroxyl radical oxidation mechanism .The order of combination ability with ct-DNA and cleaving pBR322 DNA of the two complexes is complex 2〉com-plex 1, which is in accordance with the bacteriostatic activity .
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2014年第2期90-95,共6页
Journal of South China Agricultural University
基金
华南农业大学"211工程"三期重点建设项目(2009B010100001)
