摘要
目的通过免疫组化研究CD44及CD44v6在癌前食管及食管鳞状细胞癌中的表达情况，探讨其与食管鳞状细胞癌的关系及临床意义。方法收集潮州市中心医院病理科存档的食管病例蜡块（所有病例术前均未接受放、化疗）。采用免疫组化PV-9000二步法（非生物素）检测癌前食管63例，食管鳞状细胞癌140例。SPSSl6．0统计软件分析实验结果。对于各组之间表达情况的数据统计采用多独立样本非参数检验：Kruskal．WallisH检验；各组中两两比较采用两独立样本非参数检验：Kruskal—WallisH检验的方法进行比较。结果CD44及CD44v6在正常的鳞状上皮，只表达于上皮基底层3～5层细胞，随着上皮的增生，表达的细胞层数逐渐增加，在非典型增生的上皮则主要表达于有异型的上皮，而原位癌则几乎表达于上皮的全层。CD44及CD44v6在各种食管癌前病变组织与食管鳞状细胞癌中的表达均存在差异（均P〈0．01），其中从正常食管组织及伴上皮增生但无细胞异型的食管组织至食管鳞状细胞癌之间，其表达强度逐渐增强，而从非典型增生及原位癌至食管鳞状细胞癌之间的表达强度逐渐减弱。结论CD44及CD44v6在癌前食管及食管鳞状细胞癌中的表达差异，提示它们可能与食管鳞状细胞癌的发生及发展有关，为食管癌干细胞的进一步研究提供线索。
Objective To explore the relationship and clinical significance through investigated the expression of CD44 and CD44v6 in different types of esophageal epithelial hyperplasia and squamous cell carcinoma by immunohistochemical method. Methods Paraffin blocks of esophageal carcinoma were collected from department of Pathology, Chaozhou central hospital. The patients were not treated with chemotherapy or radiotherapy before operation. The expression of CD44 and CD44v6 in 63 cases of esophageal epithelial hyperplasia and 140 cases of esophageal squamous cell carcinoma （ESCC） were detected by immunohistochemistry PV-9000 two-steps methods using monoclonal antibodies. The results were analyzed with SPSS 16.0 statistics software. The difference of expression between each sample in one group was analyzed by multi-independent samples nonparameter tests （Kruskal-Wallis H tests）. The difference of expression between two samples in one group was analyzed by 2-independent samples nonparameter tests （Kruskal-Wallis H tests）. Results In normal squamous epithelium, only 3-5 layers of cells were positive for CD44 and CD44v6, and the expression of cell layers gradually increased with the epithelial hyperplasia. The atypical hyperplasia epithelium were mainly expressed in atypia epithelial, and almost all epithelial layers expressed in carcinoma in situ. There were significant difference for the expression of CD44 and CD44v6 between normal squamous epithelium and ESCC, the hyperplasia epithelium and ESCC, the atypical hyperplasia epithelium and ESCC, the carcinoma in situ and ESCC （all P〈0.01）. The expression of CD44 and CD44v6 in ESCC was greater than normal squamous epithelium and hyperplasia epithelium, but it was weaker than atypical hyperplasia epithelium and carcinoma in situ. Conclusion The different expression of CD44 and CD44v6 in different types of esophageal epithelium and ESCC implied that CD44 and CD44v6 may be relate to the occurrence and development of ESCC. They may be the stem cells of ESCC.
出处
《分子诊断与治疗杂志》
2013年第4期236-239,共4页
Journal of Molecular Diagnostics and Therapy
基金
广东省医学科学技术研究基金(B2008179)
广东省社会发展计划(2011B031800329)
