摘要
建立同时测定小根蒜中尿苷、鸟苷和腺苷含量的反相高效液相色谱(RP-HPLC)梯度洗脱法。采用ZORBAX-SB-C18色谱柱(150 mm×4.6 mm,5μm);以水-甲醇为流动相,梯度洗脱,流速为0.6 mL/min,柱温为25℃;检测波长:260 nm。尿苷在0.0784-0.784μg范围内线性关系良好,线性相关系数r=0.9995;鸟苷在0.0798-0.798μg范围内线性关系良好,线性相关系数r=0.9995;腺苷在0.0852-0.852μg范围内线性关系良好,线性相关系数r=0.9994。该法操作简便,方法可靠,重现性好,可以作为小根蒜的质量控制方法之一。
A new method for determination of uridine, guanosine and adenosine contents in Allium macrostemon bunge were established by using RP-HPLC gradient elution. The extracts were separated on a ZORBAX-SB-C18 analytical column （4.60 mmx250 mm, 5 lain） with CH3OH-H20 as mobile phase at 260 nm. The flow rote was 0.6 mL.min land the column temperature was 25 C. The linear ranges of uridine,guanosine and adenosine were 0.0784-0.784 μg （r=0.9995）, 0.0798-42.798 μg （r=0.9995） and 0.0852~0.852 μg （r=0.9994）, respectively. This method showed high reproducibility and can be used for quality control of the Allium macrostemon bunge.
出处
《现代食品科技》
EI
CAS
北大核心
2013年第5期1128-1130,1191,共4页
Modern Food Science and Technology
基金
贵州省科技创新人才团队项目(合同号:黔科合人才团队(2010)4011号)
关键词
高效液相色谱法
小根蒜
尿苷
鸟苷
腺苷
HPLC
allium macrostenon bunge
uridine
guanosine
adenosin
