摘要
为研究有机溶剂对分蘖葱头中有机硫化合物提取及成分的影响,采用二氯甲烷、乙酸乙酯、正己烷3种溶剂提取分蘖葱头中有机硫化合物,并利用顶空固相微萃取和气相色谱-质谱联用技术对不同溶剂提取到有机硫化合物进行比较分析。结果表明:二氯甲烷提取分蘖葱头中有机硫化合物的最佳工艺条件为浸提时间4 h、料液比1∶4(g/m L)、搅拌速率250 r/min,在此条件下有机硫化合物得率为(0.413 3±0.007)%。乙酸乙酯、正己烷有机硫化合物得率分别为(0.273 3±0.011)%、(0.288 9±0.009)%。3种溶剂共提取分离出45种组分,均检测出9种有机硫化合物,其中相对含量较高的均为噻吩类和硫醚类。另外,以二氯甲烷为溶剂提取出独有的有机硫化合物12种;以乙酸乙酯为溶剂提取出独有的有机硫化合物3种;以正己烷为溶剂提取出独有的有机硫化合物8种。
In this study,we examined the effects of different organic solvents on the extraction efficiency and compositionof organic sulfur-containing compounds from tillering onion.The organic sulfur-containing compounds were extracted bydichloromethane and ethyl acetate and n-hexane,respectively,and then analyzed by headspace solid phase microextractionand gas chromatography-mass spectrometry(HS-SPME-GC-MS).The optimum extraction conditions with dichloromethaneas the extraction solvent were determined to be4h extraction at a ratio of material to liquid of1:4(g/mL)with stirring at250r/min,leading to a yield of(0.4133±0.007)%,which was higher than those obtained with ethyl acetate and n-hexaneas the extraction solvent,(0.2733±0.011)%and(0.2889±0.009)%,respectively.A total of45components were extractedby the three solvents and9organic sulfur-containing compounds were detected in each solvent extract,among which,thiophenes and thioethers were predominant.In addition,12,3and8unique organic sulfur-containing compounds wereextracted by methylene chloride,ethyl acetate,and n-hexane.
作者
刘婷婷
秦宇婷
吴玉莹
张晶
张艳荣
LIU Tingting;QIN Yuting;WU Yuying;ZHANG Jing;ZHANG Yanrong(College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2017年第12期151-156,共6页
Food Science
基金
吉林农业大学人才计划项目(20160509)
关键词
有机溶剂提取
分蘖葱头
有机硫化合物
气相色谱-质谱联用
organic solvent extraction
tillering onion
organic sulfur-containing compounds
gas chromatography-mass spectrometry
