摘要
目的对小根蒜中甾体皂苷类成分进行分离和结构鉴定,并对得到的化合物进行体外抗癌活性评价。方法使用大孔吸附树脂、硅胶、ODS柱色谱及制备高效液相色谱等方法,通过化合物的理化性质及各种光谱技术鉴定化合物的结构。采用MTT法测定化合物体外抗癌活性。结果从小根蒜60%(φ)乙醇提取物中分离得到8个甾体皂苷类化合物,分别鉴定为薤白皂苷B(1)、薤白皂苷C(2)、薤白皂苷E(3)、(22S)胆甾-5-烯-1-β,3-β-,16β,22羟基1氧-α-L-鼠李吡喃糖基16-氧-β-D-葡萄吡喃糖苷(4)、(25R)26氧βD葡萄吡喃糖基22羟基呋甾5(6)烯3β,26二醇3氧βD葡萄吡喃糖基(1→2)[βD葡萄吡喃糖基(1→3)]-β-D-葡萄吡喃糖基(1→4)-β-D-半乳吡喃糖苷(5)、薤白皂苷F(6)、薤白皂苷G(7)、(25R)26氧-β-D-葡萄吡喃糖基22羟基-5-β-呋甾-3β,26-二醇-3-氧-β-D-葡萄吡喃糖基(1→2)-β-D-半乳吡喃糖苷(8)。化合物2、5、8在25μg·mL-1的质量浓度下可以明显地抑制SF268和NCIH460肿瘤细胞的生长。结论化合物8为从本属植物中首次分离得到,化合物4、5为首次从中药小根蒜中分离得到,化合物2、5、8有明显的体外抗癌作用。
Aim To isolate and structurally elucidate the steroidal saponins of Allium macrostemon Bunge and evaluate the anti-cancer bioactivities of these compounds in vitro.Methods Solvent extraction and column chromatography were used to isolate the steroidal saponins and physic-chemical constants and spectroscopic analysis were employed for the structural elucidation.MTT were used to evaluate the bioactivities in vitro.Results Eight steroidal saponins were isolated and elucidated as macrostemonoside B(1),macorstemonoside C(2),macorstemonoside E(3),(22S)-cholest-5-ene-1β,3β,16β,22-tetraol-1-O-α-L-rhamnopyranosyl-16-O-β-D-glucopyranoside(4),(25R)-26-O-β-D-glucopyranosyl-22-hydroxy-furost-5(6)-ene-3β,26-diol-3-O-β-D-glucopyranosyl(1→2)[β-D-glucopyranosyl(1→3)]-β-D-glucopyranosyl(1→4)-β-D-galactopyranoside(5),macorstemonoside F(6),macorstemonoside G(7),(25R)-26-O-β-D-glucopyranosyl-22-hydroxy-5β-furost-3β,26-diol-3-O-β-D-glucopyranosyl(1→2)-β-D-galactopyranoside(8).Compounds 2,5 and 8 could extremely inhibit the growth of cancer cell SF-268 and NCI-H460 at the concentration of 25 μg·mL -1.Conclusion Compounds 4,5 and 8 are isolated from Allium macrostemon Bunge for the first time and compounds 2,5 and 8 show anticancer bioactivities in vitro.
出处
《中国药物化学杂志》
CAS
CSCD
2005年第3期142-147,共6页
Chinese Journal of Medicinal Chemistry
关键词
药物化学
结构鉴定
柱色谱法
抗癌活性
MTT法
小根蒜
甾体皂苷
medicinal chemistry
structural identification
column chromatography
anticancer bioactivities
MTT
Allium macrostemon Bunge
steroidal saponins
