摘要
目的:优选大孔吸附树脂纯化薄荷总酚酸的工艺条件。方法:以总酚酸含量和吸附-解吸率为指标,考察5种不同大孔吸附树脂对总酚酸的吸附能力,确定最佳树脂型号,并优化其纯化工艺条件。结果:选用HPD-400型大孔吸附树脂,最佳工艺条件为上样液质量浓度0.1 g.mL-1,上样液pH 3,上样量6 BV,树脂柱径高比1∶9,2 BV 20%乙醇洗脱除杂,流速2BV.h-1,3 BV 70%乙醇以4 BV.h-1流速洗脱,收集洗脱液,薄荷总酚酸纯度可达53%。结论:所建立的工艺分离效果良好,操作简单,重复性好,可用于分离富集薄荷总酚酸。
Objective： To optimize purification technology of total phenolic acid from Mentha macroporous resin. Method： With the content of total phenolic acid and adsorption-resolution rate as indexes,optimum resin model was determined by investigating adsorption capacity of five different macroporous resin on total phenolic acid, and optimized its purification technology conditions. Result： HPD-400 macroporous resin was selected, Optimum purification conditions were as follow： The concentration of sample liquid was 0. 1 g ·mL^-1 and then turn to pH 3 with acetic acid,the amount of sample 6 BV, ratio of diameter-height 1 ： 9, 2 BV 20% ethanol to wipe off impurity with flow velocity of 2 BV·h^-1,3 BV 70% ethanol to elute with 4 BV ·h ^-1, eluate collected, purity of total phenolic acid 53%. Conclusion： Optimized purification technology was fit for separating and easy to operate with good reproducibility, it could be used to separate and enrichment total phenolic acid from M. haplocalyx.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第16期9-11,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
高等学校博士学科点专项科研基金(20100013110002)
国家自然科学基金项目(81173520)
关键词
薄荷
总酚酸
大孔吸附树脂
纯化
Mentha haplocalyx
total phenolic acid
macroporous adsorption resin
purification
