摘要
目的:优选大孔吸附树脂纯化丹参水溶性有效成分的工艺条件。方法:采用高效液相色谱法,以丹酚酸B的含量为指标,对大孔吸附树脂型号、吸附条件、洗脱条件进行了考察。结果:最佳工艺条件为采用XDA-5型大孔吸附树脂,控制药液浓度以丹酚酸B计约为18 mg/ml,pH=4.0,吸附过柱,以70%乙醇3倍柱体积洗脱,丹酚酸B洗脱率在90%以上。结论:优选工艺可较好的纯化富集丹参水溶性有效成分。
Objective： To study the optimum conditions for the isolation and purification of water-soluble substance from Salvia miltiorrhiza Bge.. Methods： The optimum macroporous resin was selected and the separation and purification process was evaluated by measuring the content of Salvianolic acid B in the fractions by HPLC. Results： The XDA-5 macroporous resin was the most effective compared with other macroporous resins. The optimum conditions were screened, which were 18 mg/ml corresponding to Salvianolic acid B for concentration of extract, pH was 4, and the volume of 70% （V/V） ethanol as eluant was 3 BV. By this method, the elution efficiency of Salvianolic acid B exceeded 90%. Conclusion： The method is more effective for large-scale isolation and purification of water-soluble substance from Salvia miltiorrhiza Bge..
出处
《中药材》
CAS
CSCD
北大核心
2007年第10期1308-1310,共3页
Journal of Chinese Medicinal Materials
基金
广州市科技计划项目(2005Z3-E5041)
关键词
丹参
大孔吸附树脂
丹酚酸B
Salvia miltiorrhiza Bge.
Macroporous resins
Salvianolie acid B
