摘要
目的:观察肿瘤坏死因子相关凋亡诱导配体(TRAIL)与紫杉醇联合对鼻咽癌CNE-1和CNE-2细胞株的生长抑制作用及凋亡诱导效应。方法:TRAIL和紫杉醇分别单独作用及联合作用于CNE-1和CNE-2细胞后,CCK-8法检测其生长抑制率,流式细胞术检测其凋亡率。结果:TRAIL和紫杉醇对CNE-1和CNE-2细胞均具有抗增殖作用,且在一定范围内呈时间-剂量依赖性;TRAIL与紫杉醇联合应用于CNE-1和CNE-2细胞,其生长抑制率和凋亡率均大于二者单独应用时细胞生长抑制率和凋亡率,差异有统计学意义(P<0.05)。结论:TRAIL和紫杉醇联合作用于鼻咽癌细胞,其生长抑制率和凋亡率显著高于二者单独用药,两药联合应用具有协同杀伤效应,明显优于单独用药。
Objective：To investigate the synergistic cytotoxicity of TRAIL and paclitaxel on nasopharyngeal cell lines CNE-1 and CNE-2.Method：CCK-8 assays the growth inhibition rate of CNE-1 and CNE-2 which was treated with TRAIL or paclitaxel or conbination of both.Flow cytometry tests the apoptosis rate of CNE-1 and CNE-2 which was treated with TRAIL or paclitaxel or conbination of each other.Result：In certain range of time and concentration,TRAIL and paclitaxel inhibited the growth of the cell lines of CNE-1 and CNE-2 in a time-dose dependent manner（P0.05）.The rate of growth inhibition and apoptosis in TRAIL and paclitaxel combinative group was more significant than that in the TRAIL and paclitaxel singular group（P0.05）.Conclusion：TRAIL and paclitaxel had a synergistic killing effect on NPC cell lines and showed better effection than singular group,which provides a novel and prospective strategy for NPC chemotherapy.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2012年第7期319-322,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基金
高等学校博士学科点专项科研基金课题(No:20090162110065)
湖南省科技厅项目(No:2007SK2003)
2010年湖南省研究生学位论文创新基金资助(No:CX2010B053)
