摘要
目的建立大鼠下牙槽神经血管束离断和牙周炎双重动物实验模型,从组织学上观察神经损伤对牙周炎进展的影响。方法将36只2月龄SPF级Wistar雄性大鼠随机分为3组,每组12只。①对照组:结扎左侧下颌第一磨牙,喂10%白砂糖水,行饮食诱导;②实验组:结扎左侧下颌第一磨牙,左侧下牙槽神经离断,喂10%白砂糖水,行饮食诱导;③假手术组:结扎左侧下颌第一磨牙,左侧假手术操作,喂10%白砂糖水,行饮食诱导。分别在第2、4、6、8周取标本,行HE切片观察牙周组织的变化。结果从X线检查结果和组织学结果看,实验组牙周炎症程度明显重于对照组和假手术组,对照组和假手术组没有明显差异。结论离断下牙槽神经血管束对牙周炎的进展有明显的促进作用。
Objective To establish the animal modal of the inferior alveolar nerve（IAN）transected and experimental periodontitis in rats,exploring the funtion of nerve factor in the occurrence and development of periodontitis in histological level.Methods 36 SPF Wistar male rats（2 months of age）were divided into three groups randomly.In the control group and the sham surgical of the IAN-transection,steel-wire were given to the gingivas around the left of first maxillary molars.After the surgery,the rats were feeded with 10% sugar solution to drink and chew softened with that solution.In the experimental group,steel-wire were given to the gingivas around the left of first maxillary molars,the gingiva was lacerated,the surgical transection of the IAN-transection was applied to the left of the experimental group.After the surgery,the rats were provided with 10% sugar solution to drink and chew softened with that solution every day.The rats were killed at the second,fourth,sixth and eighth week and routine HE histological section was performed to observe the change of the periodontal tissue.Results Histological examination and the X-radiography showed that the damage of periodontal tissue in the IAN-transection side overweight the control group and the sham surgical of the IAN-transection obviously.The control group and the sham surgical group of the IAN-transection were no significant differences.Conclution The inferior alveolar nerve（IAN） transected may have a positive influence on the occurrence and development of periodontitis.
出处
《滨州医学院学报》
2011年第6期422-426,共5页
Journal of Binzhou Medical University
基金
山东省自然科学基金(No.ZR2010HM050)
山东大学自主创新基金(No.2009TS048)
