摘要
目的探讨表皮生长因子受体(EGFR)蛋白在宫颈鳞状上皮内瘤变和鳞状细胞癌中的表达及基因扩增,并评估其在宫颈癌发生、发展和预后中的价值。方法分别应用免疫组化EnVision法和荧光原位杂交(FISH)技术检测75例宫颈各种病变组织中的EGFR蛋白表达、基因扩增及基因拷贝数的变化,其中慢性宫颈炎10例,低级别鳞状上皮内瘤变(CIN)16例,高级别CIN 25例,宫颈癌24例。结果 EGFR在高级别CIN和宫颈癌中的阳性表达率分别是76%和79.17%,而在慢性宫颈炎和低级别CIN中的阳性表达率分别是10%和43.75%。高级别CIN和鳞癌中的基因扩增阳性率为25%,而慢性宫颈炎和低级别CIN中的阳性率为3.33%。42例EGFR阴性或低等阳性病例中FISH检测出26例(61.9%)二倍体和11例(26.2%)三倍体,而20例EGFR中等或强阳性病例中却有13例(65%)为多倍体或单纯EGFR基因扩增;所有EGFR基因扩增病例的蛋白表达均为中～强(+)。结论 EGFR可能参与了宫颈癌的发生,并且是宫颈癌发生的早期事件。EGFR蛋白过表达可能由EGFR基因扩增或基因拷贝数增加所致。
Objective To study the protein expression and gene amplification of epithelial growth factor receptor（EGFR） in intraepithelial neoplasias and squamous cell carcinoma of cervix and to explore the value of EGFR in the carcinogenesis,progression and prognosis of cervical squamous cell carcinoma.Methods EGFR protein expression and gene amplification were evaluated by immunohistochemical and fluorescence in situ hybridization（FISH） techniques,respectively,in 75 cases of various cervical lesions,including 10 chronic cervicitis,16 low grade intraepithelial neoplasias（CIN）,25 high grade CIN and 24 invasive squamous cell carcinomas.Results Expression of EGFR was observed in 76% high grade CIN and 79.17% invasive carcinoma,respectively.In contrast,there were low levels of EGFR expression in chronic cervicitis（10%） and low grade CIN（43.75%）.There were statistical differences among them（P 0.05）.Gene amplification was detected in 25.00% high grade CIN and invasive carcinoma,but there were only 3.33% EGFR gene amplification in chronic cervicitis and low grade CIN.Among the 42 patients with a negative or low level of EGFR expression,26 patients（61.90%） had diploidy and 11 patients（26.20%） had balanced trisomy,whereas among 20 patients with an intermediate and high levels of EGFR protein expression,13 patients（65.00%） had balanced polysomy or gene amplification.All the EGFR gene amplification cases had intermediate and high levels of protein expression.Conclusion EGFR alteration may involve the carcinogenesis of the cervix,which may be an early event during the carcinogenesis.Overexpression of EGFR protein may result from gene amplification and gene copy number increases.
出处
《诊断病理学杂志》
CSCD
2010年第6期448-452,共5页
Chinese Journal of Diagnostic Pathology
