摘要
目的研究黄芪总苷(Astragaloside,AST)对血管性痴呆小鼠学习记忆功能的影响,初步探讨其可能的作用机制。方法采用双侧颈总动脉反复缺血-再灌注加尾端放血的方法制备血管性痴呆小鼠模型。随机分为假手术组、模型组、丹参40 mg.kg-1、AST(40、20、10 mg.kg-1)组,术后连续ig给药21 d,末次给药后1 h,采用跳台实验和避暗实验观察各组小鼠的学习记忆功能;取胸腺和脾脏计算脏器指数;化学比色法检测脑组织MDA、NO含量和SOD、NOS活性。结果与模型组比较,各用药组均可明显改善跳台实验中各组小鼠的学习记忆能力;AST缩短避暗实验中小鼠的潜伏期,减少错误次数;与模型组比较,AST明显提高SOD活性,降低MDA、NO含量及NOS活性。结论AST能够提高血管性痴呆小鼠学习记忆功能,其机制可能与其提高脑组织的抗氧化能力有关。
Aim To observe the effect and mechanism of astragaloside（AST） on the disturbance of learning and memory of vascular de- mentia mice. Methods The animal model of the disturbance of learning and memory of vascular dementia （VD） mice was established by three repeated times ischemia-reperfusion through the ligation of the bilateral common carotid arteries. After 14d administration of AST, t he abilities of learning and memory of mice were investigated by step-down test and step-through test in all animals. The changes of thy- mus index, spleen index, MDA, SOD, NO and NOS were determined. Results Compared with the sham group, the grades of learning and memory of mice in model group were lower. The AST group could increase grades of learning and memory of mice and decrease eclipse period and wrong times. There is no difference between groups. Compared with the model group, the activity of SOD was higher, while the NO, NOS and MDA increased in AST groups. Conclusion AST may have protective effect on the disturbance of learning and memory of vascular dementia mice and the mechanism might be related to its antioxidant activity.
出处
《安徽医药》
CAS
2009年第9期1018-1020,共3页
Anhui Medical and Pharmaceutical Journal
基金
安徽省教育厅自然科学基金(NoKJ2008B301)
安徽省高等学校青年教师科研资助项目(No2008jq1059zd)
关键词
黄芪总苷
血管性痴呆
跳台实验
避暗实验
抗氧化
astragaloside
vascular dementia
step-down test
step-through test
antioxidant
