摘要
目的探讨醒脑启智胶囊对小鼠脑组织海马细胞增殖及凋亡的影响。方法用双侧颈总动脉结扎反复脑缺血再灌注的方法制备血管性痴呆小鼠模型。术后15天进行行为学实验和脑组织的病理形态学观察,并用流式细胞术检测各组海马细胞增殖指数及凋亡率的变化。结果模型组小鼠学习成绩与记忆成绩下降,各治疗组小鼠的学习成绩与记忆成绩均有提高。光镜下模型组小鼠脑组织海马区呈缺血性病理改变,各治疗组小鼠病变轻于模型组。流式细胞术检测模型组小鼠海马细胞增殖指数及凋亡率明显增高;各用药组均低于模型组。结论醒脑启智对血管性痴呆小鼠有明显治疗作用,其作用机理可能是抑制海马细胞过度增殖及凋亡。
Objective： To investigate the therapeutic effects on vascular dementia and influence on proliferation and apoptosis of hippocampus cell of Xingnaoqizhi capsule （XNQZ） in mice. Methods： The model of vascular dementia mice were duplicated with the operation of cerebral ischemic reperfusion. On the 15th day after praxiology experiment, the morphologic changes of cerebral tissue were observed as well as the proliferation index（PI） and apoptosis rate（AR） of the hippocampus cells in mice were determined by flow cytometry（FCM）. Results： ① Results of praxiology experiment shows that the learning and memorizing performance record of model group were failed and that of other groups were improved（ P 〈0.05or P 〈0.01 ）.②The pathomorphology indicates that hippocampus of mice brain tissue taking up the pathological changes of ischemia. The pathological changes of each medical group was lighter than that of model group. ③FCM shows that the PI and AR of the hippocampus cells in mice of model group were higher than those of sham-operation group（ P 〈 0.01 ）, and those of each medical group was lower than those of model group（ P 〈0.05 or P 〈0.01 ）. Conclusion： XNQZ capsule could restrain the apoptosis and the proliferation of the hippocampus cells to treat vascular dementia.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2005年第6期61-63,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
河北省科技攻关计划课题(01276207D)
